My watch list
my.bionity.com  
Login  

FRET Imaging Reveals Different Cellular Entry Routes of Self-Assembled and Disulfide Bonded Polymeric Micelles

Although nanocarriers hold promise for cancer chemotherapy, their intracellular drug delivery pathways are not fully understood. In particular, the influence of nanocarrier stability on cellular uptake is still uncertain. By physically loading hydrophobic FRET probes, we revealed different intracellular drug delivery routes of self-assembled and disulfide bonded micelles. The self-assembled micelles were structurally dissociated by micelle–membrane interactions, and the hydrophobic probes were distributed on the plasma membrane. Alternatively, intact disulfide bonded micelles carrying hydrophobic probes were internalized into cancer cells via multiple endocytic pathways. Following internalization, disulfide bonded micelles were decomposed in early endosomes by glutathione-mediated disulfide bond reduction, exposing the probes to intracellular organelles.

Authors:   Seung-Young Lee; Jacqueline Y. Tyler; Sungwon Kim; Kinam Park; Ji-Xin Cheng
Journal:   Molecular Pharmaceutics
Year:   2013
DOI:   10.1021/mp4003333
Publication date:   15-Aug-2013
Facts, background information, dossiers
More about American Chemical Society Publications
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE