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ABSTRACT
Sapovirus (SaV), a member of the family Caliciviridae, is an important cause of acute epidemic gastroenteritis in humans. Human SaV is genetically and antigenically diverse, and it can be classified into four genogroups (GI, GII, GIV, and GV) and 16 genotypes (7 GI [GI.1–7], 7 GII, [GII.1–7], 1 GIV and 1 GV), based on capsid sequence similarities.
Monoclonal antibodies (MAbs) are powerful tools for examining viruses and proteins. PAI myeloma cells were fused with spleen cells from mice immunized with a single type of recombinant human SaV virus‐like particles (VLPs) (GI.1, GI.5, GI.6, GII.3, GIV, or GV). A total of 65 hybrid clones producing MAbs were obtained. With an ELISA, 24 MAbs were characterized, according to their cross‐reactivity to each VLP (GI.1, GI.5, GI.6, GII.2, GII.3, GII.4, GII.7, GIV, and GV). With this method, the MAbs were classified into: 1) MAbs broadly cross‐reactive to all GI, GII, GIV and GV strains, 2) those reactive in a genogroup‐specific, and 3) those reactive in a genotype‐specific manner. Three broadly cross‐reactive MAbs were further analyzed with a competitive ELISA that demonstrated at least two different common epitopes are located on the capsid protein of human SaVs in the four genogroups.
The MAbs generated and characterized in this study will be useful tools for further study of the antigenic and structural topography of the human SaV virion and for developing new diagnostic assays for human SaV.
| Authors: | Noritoshi Kitamoto, Tomoichiro Oka, Kazuhiko Katayama, Tian‐Cheng Li, Naokazu Takeda, Yoji Kato, Tatsuya Miyoshi, Tomoyuki Tanaka | |
| Journal: | Microbiology and Immunology | |
| Year: | 2012 | |
| Pages: | no | |
| DOI: | 10.1111/j.1348-0421.2012.00499.x | |
| Publication date: | 25-08-2012 |
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