My watch list
my.bionity.com  
Login  

A non-rotational, computer-controlled suspension bioreactor for expansion of umbilical cord blood mononuclear cells

The proliferation and differentiation characteristics of umbilical cord blood mononuclear cells were examined in a non-rotational suspension bioreactor with a fishtail mixer. The system consisted of a glass vessel, a mixer that moved vertically, a data acquisition and control system to continuously monitor pH, temperature and dissolved O2. The bioreactor provided superior expansion of total HSCs and not total cell number, as well as expression of stemness-related genes which followed with increasing in number of colony-forming cells during 14 days of culture compared to T -lask culture. Vertical agitation thus reduces the total cell number, which may be related to increased shear stress, but has no effect on HSC function.

Authors:   Niloufar Shayan, Marzieh Ebrahimi, Bahareh Beiki, Ehsan Janzamin
Journal:   Biotechnology Letters
Year:   2012
DOI:   10.1007/s10529-012-0993-5
Publication date:   10-07-2012

Watchlist

This is where you can add this publication to your personal favourites.

Additional Information

Facts, background information, dossiers
More about Springer-Verlag
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE