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In this work, the in-house and interlaboratory validation of a DNA extraction method from pollen in an unifloral rape honey as well as several multifloral honeys is described. The amplifiability of plant and rape DNA amplifiable by real-time PCR was used as a parameter for the evaluation of the method. The practical (i.e., relative) limit of detection was used as a tool for assessing the suitability of the extraction method for further GMO analysis. In a collaborative study with 14 participating labs the results of the in-house validation could be confirmed. The amount of amplifiable plant and rape DNA varied depending on the type of honey. For rape honey, a mean practical LOD of 0.12 % was obtained.
| Authors: | Hans-Ulrich Waiblinger, Marc Ohmenhaeuser, Stefanie Meissner, Miriam Schillinger, Klaus Pietsch, Ottmar Goerlich, Joachim Mankertz, Kathrin Lieske, Hermann Broll | |
| Journal: | Journal für Verbraucherschutz und Lebensmittelsicherheit | |
| Year: | 2012 | |
| DOI: | 10.1007/s00003-012-0774-z | |
| Publication date: | 31-05-2012 |
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