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The VP6 protein of rotavirus A (RVA) is a target antigen used for diagnostic assays and also for the development of new RVA vaccines. We have compared the expression of VP6 protein in human embryonic kidney (HEK293-T) cells with results obtained using a well-established insect cell-baculovirus system. The recombinant VP6 (rVP6) expressed in HEK293-T cells did not present degradation and also retained the ability to form trimers. In the insect cell-baculovirus system, rVP6 was expressed at higher levels and with protein degradation as well as partial loss of ability to form trimers was observed. Therefore, HEK293-T cells represent a less laborious alternative system than insect cells for expression of rVP6 from human RVA.
| Authors: | Haroldo Cid da Silva Junior, Sérgio da Silva e Mouta Junior, Marcos César Lima de Mendonça, Mirian Claudia de Souza Pereira, Alanderson da Rocha Nogueira, Maria Luiza Borges de Azevedo, José Paulo Gagliardi Leite, Márcia Terezinha Baroni de Moraes | |
| Journal: | Biotechnology Letters | |
| Year: | 2012 | |
| DOI: | 10.1007/s10529-012-0946-z | |
| Publication date: | 11-05-2012 |
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