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Abstract
We performed a proof‐of‐concept study to determine if human pathogens could be detected in clinical specimens using nanolitre‐volume real‐time PCR. Nanolitre PCR for Bordetella pertussis/B. parapertussis and respiratory syncytial virus (RSV) was performed on nasopharyngeal specimens and results compared to conventional methods. B. pertussis /B. parapertussis nanolitre PCR detection was 100% sensitive (20/20, 95% CI 84% ‐ 100%) and 100% specific (26/26, 95% CI 87% ‐ 100%). RSV nanolitre PCR was also 100% sensitive (21/21, 95% CI 85% ‐ 100%) and specific (25/25, 95% CI 87% ‐ 100%). Respiratory pathogens can be successfully detected in clinical specimens using nanolitre‐volume PCR.
© 2012 The Authors Clinical Microbiology and Infection © 2012 European Society of Clinical Microbiology and Infectious Diseases
| Authors: | Slinger, Robert; Moldovan, Ioana; Barrowman, Nicholas; Chan, Francis | |
| Journal: | Clinical Microbiology and Infection | |
| Year: | 2012 | |
| Pages: | no | |
| DOI: | 10.1111/j.1469-0691.2012.03896.x | |
| Publication date: | 16-04-2012 |
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