The Arabidopsis thaliana PRP39, encoding a tetratricopeptide repeat protein, is a novel gene that affects flowering time via an autonomous pathway. In this study, a PRP39 homolog gene was isolated from Doritaenopsis ‘Tinny Tender’ (Doritaenopsis Happy smile × Happy valentine) and designated as DhPRP39. The full-length DhPRP39 cDNA was 2,962 bp long with a 2,472 bp open reading frame and encodes 823 amino acids. The putative DhPRP39-encoded protein contained four conserved, three half-a-tetratricopeptide repeat, and one RNA14 regions. Amino acid sequence alignment showed that DhPRP39 shares a high similarity to PRP39 homologs from other species. Real-time quantitative reverse transcription polymerase chain reaction analysis showed that DhPRP39 is ubiquitously expressed in vegetative and reproductive organs. The transcripts of this gene reached higher levels in the vegetative organs (roots, stems, and leaves) during the transition from vegetative to reproductive growth, in which the stems exhibited the strongest expression. DhPRP39 was overexpressed in Arabidopsis thaliana, and flowering of these transgenic plants were much delayed by 6–7 days compared with wild-type Arabidopsis. DhPRP39 may play an important role in the regulation of plant flowering.
Xiaoming Sun, Qiaoping Qin, Jing Zhang, Chi Zhang, Mingbing Zhou, Kee Yoeup Paek, Yongyi Cui
Endothelial nitric oxide synthase polymorphism G298T in association with oxidative DNA damage in coronary atherosclerosis
Content Type Journal Article
Category Research Note
RAJESH G. KUMAR, Department of Genetics, Osmania Univers ... more
A micropropagation protocol was developed for multiplication of seedless lemon (Citrus limon L. cv. Kaghzi Kalan) using nodal explants. The maximum shoot regeneration was observed on low level of BAP (0.1 mg l−1) or kinetin (0.5 mg l−1). BA was recorded to be better than kinetin in terms of ... more
The objective of this study was to evaluate the activity, kinetic behavior, and thermal inactivation kinetics of peroxidase (POD) in aqueous extracts from two kinds of milk ripe stage corn, sweet corn and waxy corn. Optimum activities using guaiacol as the hydrogen donor were obtained for s ... more