Simultaneous Analysis of 17 Steroid Hormones using (U)HPLC-MS/MS

Austrian BIOCRATES Life Sciences AG, cutting-edge developer of metabolomic kits, has launched its new steroid hormone screening assay, the AbsoluteIDQ^® Stero17 Kit, allowing a simultaneous analysis of a panel of 17 steroid hormones using tandem mass spectrometry - from a single blood sample.

What is AbsoluteIDQ^® Stero17? The AbsoluteIDQ^® Stero17 Kit covers the standardized quantitative analysis of 17 steroid hormones from only a single blood sample using (U)HPLC-MS/MS, a technology meeting the most stringent quality control criteria for routine diagnostics, ensuring a high level of analytical sensitivity and selectivity, and guaranteeing superior accuracy and excellent precision for robust results. The kit, which has been approved by proficiency tests, comes complete with isotope-labeled internal standards, reagents, and protocols for sample pre­paration based on solid-phase extraction in a 96-well plate format and subsequent (U)HPLC-ESI-MS/MS analysis in highly specific multiple reaction monitoring (MRM) mode and a tightly quality-controlled workflow.

KEY Benefits

• Ready-to-use kit
• More High Throughput with UHPLC-MS/MS
• Simplyfied and faster Sample Preparation
• Now DHT included
• Most comprehensive steroid hormone screening tool
• Flexible sample number batches
• Developed for HPLC- as well as for improved high throughput UHPLC-MS/MS analysis
• High quality standards and reliable results through QC-implemented workflow control
• Highly robust kit technology with superior analytical performance
• Extraordinary analytical precision, accuracy, selectivity and sensitivity

Steroid hormones - central to many of life’s processes. Steroid hormones play a crucial role in a wide range of physio­lo­gical processes. Glucocorticoids are not only involved in carbohydrate metabolism, they also affect the lipid and protein metabolism and play an important role in the reaction to stress. Estrogens and progestagens regulate almost all of the reproductive processes in women. Inborn or acquired disorders of steroid hormone metabolism have been the source of a number of serious diseases (e.g. Cushing syndrome, adrenal tumors, hyperaldosteronism).

This shows how significant the role of steroid hormones in the body may play and how important the quantitative analysis of steroid hormones in clinical diagnostics and research based applications can be. Particularly the main advantages of LC-MS/MS compared to immunoassays are multiplexing (simultaneous analysis of multiple target analytes), higher specificity (by so-called multiple reaction monitoring (MRM), greater dynamic range of the calibration and lower inter-laboratory variability (including diversity in sample preparation, calibrators and analytical methods).

For further information please visit our product website, download our product brochure or get in contact with us!