ELISA kits for the measurment of SDF-1/CXCL12

SDF-1α (CXCL12) is a CXC chemokine. The small basic protein (ca. 8 kDa) has no glycosylation site in its sequence. The homology properties of SDF-1α are unique among the chemokines. It has a high degree of conservation within the mammalians, with 99 % identity between the human and the murine SDF-1α sequences. It shows a weak homology to the other CXC chemokines. The SDF-1 gene is on chromosome 10, whereas the other chemokine genes are on chromosome 4 and 17. Except for SDF-1α there is another isoform of this chemokine, SDF-1β. SDF-1α and SDF-1β result from alternative splicing. SDF-1α binds to and activates the receptors CXCR4 and CXCR7, whereupon SDF-1α is the only known ligand for CXCR4. SDF-1α binds also to fibronectin-1, several glycosaminoglycans and heparin. SDF-1α is constitutively expressed by bone marrow stromal cells and many other tissues (for example heart, liver, brain, muscle, kidney). The function of SDF-1α is multifaceted. It plays a role in the prenatal development of the neural, hematopoietic and cardiovascular systems and is involved in the postnatal migration of CXCR4- and CXCR7-positive stem cells. Furthermore, it has a chemoattractant effect on CXCR4-positive lymphocytes and plays a role in the growth and the metastasis of CXCR4- and CXCR7-positive tumors. Except for the chemoattractant effects SDF-1α has an inhibiting effect on HIV-1 in vitro.

Advantages of the ibt human SDF-1 ELISA (Sandwich-ELISA):

• works in serum and plasma
• standard curve is parallel to serum dilutions
• uses fully synthetic SDF-1 (Stromal cell-derived factor-1)
• tested with interfering substances as e.g. hemoglobin, bilirubin, trigylcerides
• elevated levels have been found in sera from patients with cancer, rheuma and diabetes, so there are possible diagnostic applications of the assay

Advantages of the ibt rat and mouse ELISA kits:

• use all components of the human ELISA except standards
• standards are more stable than the standards in a competitors assay
• good parallelism with serum dilutions from rat and mouse
• antibody cross-reacts with sera from rabbit, pig, sheep.

ibt participates in the BIOSCENT (BIOactive highly porous and injectable Scaffolds controlling stem cell recruitment, proliferation and differentiation and enabling angiogenesis for Cardiovascular ENgineered Tissues) project which received funding from the European Community's Seventh Framework Programme (FP7-NMP-2007-LARGE-1) under grant agreement n° 214539.

For this project ibt GmbH has developed ELISA kits for the measurement of SDF-1/CXCL12 in human rat and mouse samples.

For further information please visit our product website, download our product brochure or get in contact with us!