ACEMBL: Leading Expression Technology for Multi-Protein Complex Production

General features of the systems:

• each consist of two acceptor and three donor vectors for manipulation of DNA in vitro and in E. coli
• vectors possess features that allow you to flexibly insert and recombine your gene(s) of interest into (multi-)gene expression cassettes before assembling your final construct used to transform E. coli.
• vectors possess features that enable an automated multi-gene construct assembly process; this helps speed up research, e.g. when analyzing the effects of gene variants on crystallization or on the kinetic properties of enzymes
• comes with bacterial strains for the propagation of the donor vectors and donor-donor fusions

MultiBac^Turbo-specific features:

• a bacmid, hosted in E.coli, for transfer of your final construct(s) into baculoviruses and subsequent trans-fection of your insect cells of choice
• Replacement of non-essential genes v-cath and chiA in MultiBac^Turbo bacmid improves protein production and reduces protein degradation.

Advantages of ACEMBL:

• express single or multiple proteins simultaneously from one construct
• assemble multi-gene cassettes easily and rapidly
• generate and test gene variants or mutants rapidly and in parallel to speed up and quickly fine-tune/adapt your gene expression project
• fuse single or multigene acceptor and donor plasmids via Cre recombination
• improve complex stability and solubility through co-expression of partnering proteins
• automate your entire multigene expression vector production process for high through-put and scale-up
• speed up and quickly fine-tune/adapt your gene expression project
• Easy blue/white screening for recombinant bacmids (MultiBac^Turbo only)
• no plaque purification necessary, zero background (MultiBac^Turbo only)

Applications MultiColi: heterologous expression of soluble multi-protein complexes for structural and functional elucidation, design of enzymes with novel properties or of engineered biocatalytic pathways, e.g. for bio-remediation, for synthesis of novel compounds, etc.

Applications MultiBac^Turbo:  heterologous expression of soluble eukaryotic multi-protein complexes for structural or functional elucidation, protein production in insect cells, design of virus-like particles for vaccine development,  baculoviral transfection of mammalian cells, antibody production in insect cells