MicroRNAs

News MicroRNAs

• MiReven receives further investment from MRCF

  MiReven, an Australian microRNA company commercialising discoveries from the Western Australian Institute for Medical Research (WAIMR) on the anti-cancer potential of miR-7, announced that it had received a further AU$500K investment from the Medical Research Commercialisation Fund (MRCF) due to the more

• Sichuan Agricultural University and LC Sciences Uncover the Epigenetics of Obesity

  In a study published in Nature Communications, researchers from Sichuan Agricultural University and LC Sciences report the miRNAome in porcine adipose and muscle tissues. The report provides a valuable epigenomic source for obesity prediction and prevention and furthers the development of pig as a m more

• Genetic regulators hijacked by avian and swine flu viruses identified: UBC study
  

  Researchers at the University of British Columbia have identified a number of tiny but powerful "genetic regulators" that are hijacked by avian and swine flu viruses during human infection.The discovery, published in the Journal of Virology, could reveal new targets for broad-spectrum antivirals to more

White papers MicroRNAs

• miRNA Extraction and Profiling on FFPE Samples
  

  RNA silencing is a mechanism of gene regulation whereby small double-stranded RNAs (dsRNAs) of 21-18 nucleotides in length cause mRNA degradation, mRNA translation, or chromatin modification. In the past years, more than 500 novel genes have been identified in animals and plants encoding small transcripts that can form small dsRNA hairpin structures. From these hairpin precursors, small 22-nt RNAs - so called microRNAs (miRNA) - are excised by dsRNA-specific endonucleases. The miRNAs are then integrated in ribonucleoprotein particles (RNPs). Most miRNAs in animals act by binding to partially complementary regions of the 3' untranslated region of their target mRNA, thereby blocking translation. more

• New Application for the LightCycler® 480 System: qPCR-based microRNA-Profiling
  

  Recent studies indicate that microRNAs (miRNAs) are mechanistically involved in the development of various human malignancies and therefore represent a promising new class of biomarkers. Quantitative PCR-based technologies are emerging as the gold standard for miRNA profiling. Here, a commercially available miRNA assay was used to measure and compare the expression levels of 154 miRNAs of seven lymph node samples using the LightCycler® 480 Instrument and a competitor real-time PCR system. We observed highly similar results for 134 miRNAs (87%) and some larger deviations (20 ­miRNAs/13%). In addition to large-scale miRNA profiling, it is also possible to use the LightCycler® 480 System in the assessment of miRNA expression in small sample sizes. We have conclusively demonstrated that miR-205 can be detected in a dose-dependent manner in a dilution series of 50, 10, 5, and 1 cell(s) of the KU19-19 cell line. more

• Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology
  

  MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18-25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA transcripts annotated. Although miRNAs represent a relatively abundant class of transcripts, their expression levels vary greatly among species and tissues. Less abundant miRNAs routinely fail detection using technologies such as cloning, northern hybridization and microarray analysis. Here, we present a real-time quantification method for accurate, sensitive, and economical detection of plant miRNAs based upon the stem-loop RT primer approach using an miRNA-specific primer coupled with a stem-loop specific Universal ProbeLibrary (UPL) probe. This approach represents a highly specific, sensitive, and easy-to-apply system to detect miRNAs. more

Publications MicroRNAs

• The 3′ UTR of FMR1 mRNA is a target of miR-101, miR-129-5p and miR-221: implications for the molecular pathology of FXTAS at the synapse

  While FMR1 is silenced in Fragile X syndrome (FXS) patients carrying the full mutation, its expression is elevated (2–8 fold) in premutated individuals. These people may develop the Fragile X-associated Tremor/Ataxia syndrome (FXTAS), a late onset neurodegenerative disorder characterized by ataxia more

• Putative tumor suppressor gene SEL1L was downregulated by aberrantly upregulated hsa‐mir‐155 in human pancreatic ductal adenocarcinoma

  Abstract Sel‐1‐like (SEL1L) is a putative tumor suppressor gene that is significantly downregulated in human pancreatic ductal adenocarcinoma (PDA). The mechanism of the downregulation is unclear. Here, we investigated whether aberrantly upregulated microRNAs (miRNAs) repressed the expression o more

• Mir‐142‐3p target sequences reduce transgene directed immunogenicity following intramuscular AAV1 vector‐mediated gene delivery

  Abstract Background Muscle represents an important tissue target for adeno‐associated virus (AAV) vector‐mediated gene transfer in muscular, metabolic or blood related genetic disorders. However, several studies demonstrated the appearance of immune responses against the transgene product afte more

Market studies MicroRNAs

• Rosetta Genomics, Ltd. (ROSG) - Financial and Strategic SWOT Analysis Review

  Rosetta Genomics, Ltd. (RGL) is a medical device company engaged in the development and commercialization of new diagnostic and therapeutic products based on group of genes known as microRNAs. The company has developed a discovery process that integrates computer-based algorithms and customized biol more

Websites MicroRNAs