Methylation

News Methylation

• Excess estrogen in pregnancy can silence BRCA1 in daughters, increasing breast cancer risk

  Excess estrogen levels during pregnancy can disable, in their daughters, a powerful breast cancer tumor suppressor gene, say researchers at Georgetown Lombardi Comprehensive Cancer Center. They found the DNA repair gene BRCA1 to be silenced in one year-old girls exposed to a high hormonal fetal envi more

• Novel gene drives development of different types of ovarian cancer

  Researchers at Mayo Clinic Cancer Center have identified a novel gene that can contribute to a woman's susceptibility for developing ovarian cancer. Researchers identified the gene, called HNF1B, through large-scale analysis of more than 16,000 women with ovarian cancer and more than 26,000 healthy more

• Novel epigenetic markers of melanoma may herald new treatments for patients

  Melanoma is the most serious form of skin cancer, diagnosed in more than 50,000 new patients in the United States annually. While the rate of incidences continues to rise, survival rate has not improved and the race is on to find the genetic and cellular changes driving melanoma and to devise new m more

White papers Methylation

• Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System
  

  The LightCycler® 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology. This proved to be a robust platform for performing MS-HRM experiments, allowing rapid and high-throughput studies of DNA methylation. more

• Frequent Switching of Polycomb Repressive Marks and DNA Hypermethylation in the PC3 Prostate Cancer Cell Line
  

  A key observation in these presented results was the magnitude of hypermethylation in PC3 cells, which occurred at almost all of the inactive CpG island genes. A second key observation was that when PRC marks were depleted, a PC3 hypermethylation was observed. One possible model that can explain these observations assumes that DNA methylation in PC3 cells sweeps all CpGs that are not physically protected (or "masked") (Figure 3). According to this "passive" methylation model, transcription initiation complexes at the TSS [RNA PolII and related chromatin marks, can serve as one masking mechanism (Figure 3a), and Polycomb repressive complexes can serve as another (Figure 3b). This view was also supported by recent data on DNA methylation patterns of mouse ESCs. It is important to note that even if Polycomb complexes mask loci from de novo DNA methylation, it is possible that PRC activity (or recruitment) is not masked by DNA methylation (Figure 3c). This may be the case in some of the de novo PRC targets we ob more

• Genome Sequencer 20 System: Breakthrough in a New Applications Age of Sequencing
  

  The Genome Sequencer 20 System is an ultra-high-throughput automated DNA sequencing system capable of carrying out and monitoring sequencing reactions in a massively parallel fashion. Since the Genome Sequencer 20 System provides a complete solution for ultra-high-throughput DNA sequencing, an individual researcher can for the first time prepare samples and sequencing reactions, generate sequence reads, and assemble genome sequence data within days. more

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