Topic Invitrogen

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News Invitrogen

• Selvita Appoints Piotr Prelich as a Vice President of Sales and Marketing

  Selvita announced the appointment of Mr. Piotr Prelich to the position of Vice President of Sales and Marketing. Mr. Prelich will be responsible for the further establishment of Selvita’s brand in the pharmaceutical preclinical outsourcing market, both in Poland and abroad, as well as for the sales more

• Invitrogen Announces Opening of Hepatocyte Supply Facility in Warrington, UK

  Invitrogen, part of Life Technologies Corporation, announced the opening of a new facility in Warrington, UK, which will supply freshly isolated human hepatocytes to researchers in Europe. Human hepatocytes isolated from liver tissue are used for many biopharmaceutical applications, by researchers more

• Life Technologies to Sell Mass Spectrometry Business to Danaher for $450 Million in Cash

  Life Technologies Corporation announced it has signed a definitive agreement to sell its ownership stake in its mass spectrometry business, operated as a joint venture, to Danaher Corporation for $450 million in cash. Danaher has also signed an agreement to acquire Life Technologies’ joint venture p more

White papers Invitrogen

• Agrisera Western Blot recommendations, troubleshooting and protocol
  

  Each antibody-antigen interaction has unique characteristics. A protocol giving good results for one antibody-antigen pair might found to be unsatisfactory for a second antibody even on the same sample. The interaction of antisera with protein epitopes in Western blot dependens upon a number of factors, all contributing to the final signal/noise ratio. Knowledge and control of those factors allows for modifications and optimization of the procedure. Polyclonal antibodies (serum or IgY-fractions from egg yolk) usually contain a number of different antibodies interacting independently with different epitopes on the target used for immunization. This number is limited for peptide-antigens but if recombinant or native proteins have been used for immunization the signal obtained has to be regarded as cumulative (=caused by several different types of antibodies present in the serum/IgY used) more

• Analysis of Prostate Tumour Cell Invasion Using BD FluoroBlokTMand FLUOstar OPTIMA
  

  The FLUOstar OPTIMA is used for reading the BD Biosciences FluoroBlok(TM) tumour cell invasion system. In this application note we show that the results are comparable to the traditional invasion assays. The introduced method is fast, saving time and labour. The FLUOstar OPTIMA is able to take multiple readings over time at a user defined temperature more

• Optimized Production of Lentivirus Using FuGENE® HD Transfection Reagent
  

  Lentiviral vectors are ideally suited vehicles for a broad range of research applications. Depending on pseudotype, lentiviruses infect a wide range of cell types, non-discriminately transducing both dividing and non-dividing cells. As opposed to other popular vector delivery systems, lentivirus stably and rapidly integrates genetic payload into the host genome allowing for long-term studies in vivo. Current lentiviral vector systems can accommodate upwards of ten kilobases of foreign DNA [1], although promoter and enhancer elements reduce the practical size of gene open-reading frames to perhaps five or six kilo­bases, which is sufficient to accommodate most genes commonly studied in the mammalian genome. more

Publications Invitrogen

• Labeling Mitochondria with MitoTracker Dyes

  INTRODUCTION Membrane-potential-dependent dyes such as Rhodamine 123, tetramethylrhodamine methyl ester (TMRM), and tetramethylrhodamine ethyl ester (TMRE) are useful as long as the mitochondrion maintains its negative membrane potential. MitoTracker is a commercially available fluorescent dye (Inv more

• Sequential deletion of Pichia pastoris genes by a self‐excisable cassette

  Abstract A rapid and convenient method is presented for unmarked gene deletions in Pichia pastoris. Cre/mutated lox system, Zeocin® (Invitrogen) resistance marker and homologous arms were spliced together by fusion PCR to generate the gene disruption cassettes (homologous region‐lox71‐Cre‐ZeoR‐lo more

• In Vitro and In Vivo Inhibition of MRP Gene Expression and Reversal of Multidrug Resistance by siRNA

  Abstract:  Clinical drug resistance to chemotherapeutic agents is one of the major hindrances in the treatment of human cancers. One mechanism by which a living cell can achieve multidrug resistance (MDR) is via the active efflux of a broad range of anti‐cancer drugs through the cellular membrane more

Market studies Invitrogen

• Stem Cell Technologies: World Market Outlook 2011-2021

  How will the stem cells market develop this decade? Our new report shows you that emerging industry and market's revenue potential from 2011 to 2021. This report covers stem cells in therapeutic applications: technologies, products and services. You will assess events, opportunities and activities more

• RNAi for Therapeutic Applications: Market Prospects 2011-2021

  How will the RNA interference (RNAi) therapies market develop this decade? Our new report shows you that emerging market's revenue potential from 2011 to 2021. You will see the commercial prospects for RNAi-based therapeutics. We aim to save you time and aid your decisions through our research, dat more

Websites Invitrogen