Hydrolysis

Products Hydrolysis

Automated Hydrolysis System cuts cost of fat determination thanks to full automation of laborious sample preparation

HYDROTHERM is the only system in the world, to fully automate the labour-intensive steps of acid hydrolysis in fat determination acc. to Weibull-Stoldt more

Videos Hydrolysis

Automated Hydrolysis System cuts cost of fat determination thanks to full automation of laborious sample preparation

The development of an automated hydrolysis system HYDROTHERM by C. Gerhardt sets new standards in fat analysis. The first system in the world, to fully automate the labour-intensive steps of acid hydrolysis including addition of hydrochloric acid – hydrolysis – dilution – rinsing – filtration – cle more

News Hydrolysis

• Novasep Process and Nutritis scale-up the production process for Nutritis’ fructose purification from substandard fruit

  Nutritis and Novasep Process, a division of Novasep, announced that, thanks to Novasep Process, Nutritis will scale up its production process for fructose from apple juice. Nutritis brought in Novasep to use the latter’s purification expertise to scale up its innovative process. Nutritis and Novase more

• Broccoli’s cancer protection activated by bacteria in lower gut

  Bacteria in the lower gut are responsible for breaking down the major cancer fighting chemical in broccoli to release its bioactive form. Of all the components present in crucifer vegetables, such as antioxidants and flavonoids, a molecule called glucoraphanin (GRP) is believed to play a major role more

• Enzymicals AG Acquires Proprietary Rights on Pig Liver Esterases (PLE) from Evonik AG

  The Enzymicals AG gained proprietary rights for the well-known recombinant pig liver esterases (PLE) from Evonik AG in Hanau. The intellectual property (IP) covers a range of PLE isoenzymes for hydrolysis of several compounds such as esters of secondary and tertiary alcohols, lactones and carboxylic more

White papers Hydrolysis

• High-Throughput and Complex Gene Expression Validation Using the Universal ProbeLibrary and the LightCycler® 480 System
  

  During the past decade, a considerable number of transcriptome profiling analyses have been reported. High-throughput approaches such as tagging-based serial analysis of gene expression (SAGE) and hybridization-based microarray technologies have been widely performed to profile gene expression [1]. However, these techniques are either laborious or costly, or may involve advanced technologies which limit the sampling depth, thus diminishing the sensitivity of analysis. Validation of gene expression profiles can be rapidly performed using an independent technique such as quantitative polymerase chain reaction (qPCR) to eliminate false results. In most laboratories, qPCR analysis has so far been performed on a small scale. more

• Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase
  

  Pseudomonas elastase (LasB) is a key drug target in various chronic and intractable infections. A library of 160 potential, in-house synthesised, elastase inhibitors were screened using a fluorescence assay with the internally quenched substrate Abz-Ala-Gly-Leu-Ala-Nba. Enzymatic kinetic measurements were done on a FLUOstar OPTIMA and Km and Vmax values were calculated for each inhibitor using the MARS Data Analysis software more

• Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System
  

  One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleo­tides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR). Ideally, a real-time multiplex PCR should be able to detect, differentiate, and provide a quantitative result for many different targets without a single target influencing the detection of one of the others (cross-talk) and without loss of sensitivity. It is evident that due to the limited number of fluorophoric labels available and the significant overlap in their emission spectra, quantification of multiplex reaction products is difficult and often not possible for more than two targets. more

Publications Hydrolysis

• Identification and characterization of endo-{beta}-N-acetylglucosaminidase from methylotrophic yeast Ogataea minuta

  In four yeast strains, Ogataea minuta, Candida parapolymorpha, Pichia anomala and Zygosaccharomyces rouxii, we identified endo-β-N-acetylglucosaminidase (ENGase) homologous sequences by database searches; in each of the four species, a corresponding enzyme activity was also confirmed in crude cell more

• Structures of the phage Sf6 large terminase provide new insights into DNA translocation and cleavage [Biochemistry]

  Many DNA viruses use powerful molecular motors to cleave concatemeric viral DNA into genome-length units and package them into preformed procapsid powered by ATP hydrolysis. Here we report the structures of the DNA-packaging motor gp2 of bacteriophage Sf6, which reveal a unique clade of RecA-like AT more

• Degradation profile and preliminary clinical testing of a resorbable device for ligation of blood vessels

  Publication date: June 2013 Source:Acta Biomaterialia, Volume 9, Issue 6 Author(s): Nina Aminlashgari , Odd V. Höglund , Niklas Borg , Minna Hakkarainen A resorbable device for ligation of blood vessels was developed and tested in vitro to reveal the degradation profile of the device and t more

Websites Hydrolysis