High Throughput Sequencing

Companies High Throughput Sequencing

GATC Biotech AG, Germany

GATC Biotech is a leading provider of sequencing services and innovative bioinformatics solutions for industry and academia worldwide. The company offers sequencing services using ABI 3730XLTM, 454’s GS FLXTM and Illumina/Solexa’s Genome Analyzer. GATC’s sequencing service portfolio is complemented more

News High Throughput Sequencing

• Columbia researchers report novel approach for single molecule electronic DNA sequencing

  DNA sequencing is the driving force behind key discoveries in medicine and biology. For instance, the complete sequence of an individual's genome provides important markers and guidelines for medical diagnostics and healthcare. Up to now, the major roadblock has been the cost and speed of obtaining more

• All genes in 1 go

  The majority of rare diseases are hereditary. But despite significant progress in genome research, in most cases their exact cause remains unclear. The discovery of the underlying genetic defect is, however, a prerequisite for their definitive diagnosis and the development of innovative approaches t more

• Complex Mixtures of Targeting Oligonucleotides Unlock the Power of Next-Generation DNA Sequencers

  A group of researchers at Harvard Medical School, Virginia Commonwealth University, Agilent Technologies Inc., Codon Devices Inc., and Stanford University has used complex mixtures of oligonucleotides in the form of oligonucleotide library synthesis to overcome a major bottleneck in realizing the po more

White papers High Throughput Sequencing

• Rare Variants of IFIH1 Protect Against Type-1 Diabetes
  

  Nejentsev and co-workers have found that rare alleles of all associated IFIH1 polymorphisms consistently protect from type-1 diabetes, whereas IFIH1 alleles carried by the majority of the population predispose to the disease. This observation suggests that variants that disrupt IFIH1 function in the host antiviral response have been negatively selected rather than positively selected, because they confer protection from type-1 diabetes. Although the mechanisms by which IFIH1 polymorphisms contribute to type-1 diabetes pathogenesis remain to be explored, the authors noted that one of the protective variants is a non-sense mutation leading to a truncated 626-amino acid protein lacking the C-terminal helicase domain (Figure 1), whereas two other protective variants localize to the conserved splice donor sites and probably disrupt normal splicing of the IFIH1 transcript. This suggests that variants, which are predicted to reduce function of the IFIH1 protein, would decrease the risk of type-1 diabetes, whereas n more

• Characterizing Environments: Recent Discoveries Enabled by Metagenomic Analysis with the Genome Sequencer FLX System

  Metagenomics is the study of genomic content in a complex mixture of microorganisms. The two primary goals of this approach are to characterize the organisms present in a sample and identify what roles each organism has within a specific environment. Metagenomics samples are found nearly everywhere, including several microenvironments within the human body, soil samples, extreme environments such as deep mines, and the various layers within the ocean. Until recently, technical and economic constraints limited the depth of analysis necessary to obtain a representative picture of microbial and viral communities, their metabolic profiles, and their adaptation dynamics. Advancements in sequencing technologies, including the introduction of massively parallel pyrosequencing with the Genome Sequencer FLX System, have enabled a tremendous groundswell of research in the field of metagenomics. Dramatic improvements in throughput and the elimination of biased cloning steps have extended the field far beyond tradition more

• Identification of a New Arenavirus Using High-Throughput Sequencing
  

  Culture, PCR, serologic, and oligonucleotide microarray analyses for a wide range of infectious agents were negative in samples from three individuals who had received organ transplants from a single donor and died of a febrile illness 4-6 weeks after transplantation. Palacios et al. used high-troughput sequencing with the Genome Sequencer FLX system to identify microbial sequences from RNA samples taken from the liver and kidney transplants in two recipients. They found 14 sequences representing an Old World arenavirus related to lymphocytic choriomeningitis viruses. more

Publications High Throughput Sequencing

• DROMPA: easy‐to‐handle peak calling and visualization software for the computational analysis and validation of ChIP‐seq data

  Chromatin immunoprecipitation with high‐throughput sequencing (ChIP‐seq) can identify genomic regions that bind proteins involved in various chromosomal functions. Although the development of next‐generation sequencers offers the technology needed to identify these protein‐binding sites, the analy more

• CistromeFinder for ChIP-seq and DNase-seq data reuse

  Summary: Chromatin immunoprecipitation and DNase I hypersensitivity assays with high-throughput sequencing have greatly accelerated the understanding of transcriptional and epigenetic regulation, although data reuse for the community of experimental biologists has been challenging. We created a dat more

• Lipid phosphatases identified by screening a mouse phosphatase shRNA library regulate T-cell differentiation and Protein kinase B AKT signaling [Immunology]

  Screening a complete mouse phosphatase lentiviral shRNA library using high-throughput sequencing revealed several phosphatases that regulate CD4 T-cell differentiation. We concentrated on two lipid phosphatases, the myotubularin-related protein (MTMR)9 and -7. Silencing MTMR9 by shRNA or siRNA resul more

Market studies High Throughput Sequencing

• DNA Sequencing and PCR Markets

  DNA sequencing and polymerase chain reaction (PCR) are two major technology platforms of value in life science research, drug discovery and clinical diagnostics. The markets for sequencing are extremely competitive, driven by rapid technological advancements and aggressive price wars. Next-generatio more

Websites High Throughput Sequencing