Green Fluorescent Protein

News Green Fluorescent Protein

• Researchers divide enzyme to conquer genetic puzzle

  Rice University researchers have found a way to divide and modify enzymes to create what amounts to a genetic logic gate. Biochemist Matthew Bennett and graduate student David Shis created a library of AND gates by mutating a protein from a bacterial virus. The well-understood protein known as T7 RN more

• A non-invasive intracellular 'thermometer' with fluorescent proteins has been created
  

  A team from the Institute of Photonic Sciences (ICFO) has developed a technique to measure internal cell temperatures without altering their metabolism. This finding could be useful when distinguishing healthy cells from cancerous ones, as well as learning more about cellular processes.Temperature c more

• UC San Diego biologists discover genes that repair nerves after injury

  Biologists at the University of California, San Diego have identified more than 70 genes that play a role in regenerating nerves after injury, providing biomedical researchers with a valuable set of genetic leads for use in developing therapies to repair spinal cord injuries and other common kinds o more

White papers Green Fluorescent Protein

• Transfection of Human Tooth-Specific Lineage Cells with FuGENE® HD Transfection Reagent
  

  Similar to other epithelial appendages, teeth form through reciprocal signaling interactions between the ectodermally derived dental epithelium and the mesenchyme. These signaling interactions program enamel matrix-secreting ameloblasts and dentin-forming odontoblasts. To study the signaling network involved in odontogenesis, we co-cultured primary human fetal dental epithelial cells and dental pulp cells to reconstitute the reciprocal signaling. more

• FuGENE® HD Transfection Reagent Provides High Transfection Efficiency in the Murine NB2a Cell Line
  

  Cell transfection is a method of gene delivery that introduces foreign DNA into a cell. It is one of the most important approaches in modern genetic and proteomic research. With intriguing properties, transfection of primary cells is required for tissue engineering and gene therapy. However, one major problem is that many ­methods do not work for neural cell lines. Here we report that FuGENE® HD Transfection Reagent provides a high level of transfection efficiency and intracellular protein expression in murine neuroblastoma cells, the NB2a cell line. An optimization test using different transfection reagents, including FuGENE® HD Transfection Reagent, FuGENE® 6 Transfection Reagent, and another liposome-mediated reagent, was performed for green fluores­cent protein (GFP) expression in NB2a cells through transient transfection. more

• Functional Analysis of Side Effects of Transfection Reagents in the Context of Bax-induced Apoptosis
  

  FuGENE® HD Transfection Reagent is well-suited for studying the impact of apoptosis-inducing proteins such as bax. Transfection with FuGENE® HD Transfection Reagent produced only minimal cytotoxic side effects while maintaining a high transfection efficiency. In contrast, cytotoxicity, and off-target effects of the other commercially available transfection reagents tested made it impossible to distinguish the impact of the bax protein from the impact of the transfection reagent itself more

Publications Green Fluorescent Protein

• Regulation of Mac-2BP secretion is mediated by its N-glycan binding to ERGIC-53

  The leguminous-type (L-type) lectin ER-Golgi intermediate compartment (ERGIC)-53, a homo-oligomeric endoplasmic reticulum (ER)-Golgi recycling protein, functions as a transport receptor for newly synthesized glycoproteins in the early secretory pathway. Although a limited subset of cargo glycoprote more

• The involvement of WT1 in the regulation of GADD45a in response to genotoxic stress

  Expression of the human GADD45a gene is increased in TK6 cells exposed to mutagens, clastogens and aneugens. It is known to be regulated through both p53-dependent and p53-independent pathways and WT1 has been implicated in both cases. This article reports an investigation into the effect that muta more

• A broadly applicable high-throughput screening strategy identifies new regulators of Dlg4 (Psd-95) alternative splicing [METHOD]

  Most mammalian genes produce multiple mRNA isoforms derived from alternative pre-mRNA splicing, with each alternative exon controlled by a complex network of regulatory factors. The identification of these regulators can be laborious and is usually carried out one factor at a time. We have develope more

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