Genetic Engineering

News Genetic Engineering

• Fast new, 1-step genetic engineering technology

  A new, streamlined approach to genetic engineering drastically reduces the time and effort needed to insert new genes into bacteria, the workhorses of biotechnology, scientists are reporting. Published in the journal ACS Synthetic Biology, the method paves the way for more rapid development of desig more

• U of M researchers develop a molecular 'calcium sponge' to tackle heart failure

  Researchers at the University of Minnesota's Department of Integrative Biology and Physiology and the Lillehei Heart Institute have utilized molecular genetic engineering to optimize heart performance in models of diastolic heart failure by creating an optimized protein that can aid in high-speed re more

• Mayo researchers develop editing toolkit for customizing zebrafish genomes

  Mayo Clinic researchers and an international team of scientists have developed a highly-efficient means of editing zebrafish genomes for research purposes, eliminating a bottleneck that has stymied biomedical scientists from using the fish as a model for human disease. The details appear in Nature.F more

White papers Genetic Engineering

• Outstanding Microarray Experimental Results Using FuGENE® HD Transfection Reagent
  

  Transfection is a method for delivering nucleic acids, either DNA or RNA, into animal cells in vitro. In applications such as protein production, the main purpose is to produce protein; consequently any effects on endogenous gene expression are generally irrelevant, unless the changes relate directly to loss of the cell's ability to produce protein, i.e., cell death. In contrast, in applications where the biochemical pathways are of interest, changes within the cell can alter the interpretation of the data. Unintended up- or down-regulation of genes within transfected cells is termed an off-target effect. This may be caused by the reagent itself, the vector, or the process of transfection. Such effects, if not carefully evaluated, may lead to misinterpretation of the transfection results and to misleading conclusions. Critically important in transfection experiments is the selection of the appropriate control. more

• Characterizing Biotherapeutics with HPLC
  

  As demonstrated here, there are a broad range of HPLC applications used in development, production, validation and release of protein therapeutics for human use. Some of the applications mentioned above are mandatory when submitting a new biopharmaceutical to approval at the regulatory agencies. The introduction of the first so-called biosimilars in Europe further increased the demand for highly efficient analysis methods. The continuous development of new stationary phases for the common modes of biochromatography, such as size exclusion, ion exchange and hydrophilic interaction chromatography support biochemists in increasing lab productivity and establishing high-throughput HPLC analysis not only for small molecules but also for large biopolymers more

• Increased productivity for downstream processing of biotherapeutics
  

  Polymer-based chromatographic resins featuring high mechanical and chemical stability and high protein binding capacities can help to increase the throughput and robustness of biopharmaceutical manufacturing processes. Advances in genetic engineering and cell culture technology have raised upstream productivity in the production of recombinant proteins. Increased titres of up to 10 g/l stress the need for highly efficient and robust downstream processes (DSP). Most industrial bioprocess development groups apply generic DSP platforms that are designed to purify various candidates of the same class of proteins applying the same process design. Besides various filtration and virus inactivation steps, they usually involve two or three chromatographic unit operations with orthogonal separation modes. The use of high capacity chromatographic resins can help to overcome the DSP bottleneck by increasing process throughput and robustness more

Publications Genetic Engineering

• Genetic engineering of rotaviruses by reverse genetics

  Abstract The rotavirus genome is composed of 11 gene segments of double‐stranded (ds)RNA. A recent breakthrough in the field of rotaviruses is the development of a reverse genetics system for generating recombinant rotaviruses possessing a gene segment derived from cloned cDNA. Although this ap more

• Transgenic resistance to Bamboo mosaic virus by expression of interfering satellite RNA

  Summary Plant genetic engineering has broadened the options for plant virus resistance and is mostly based on pathogen‐derived resistance. Previously, we have shown that interfering satellite RNA (satRNA) of Bamboo mosaic virus (satBaMV) greatly reduces Bamboo mosaic virus (BaMV) accumulation an more

• Marker‐free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii

  Summary The work applied a transgene expression method based on the replacement of an inactive rbcL gene as the selection marker in Chlamydomonas reinhardtii chloroplasts. The native rbcL gene in strain CC2653 has a point mutation that causes early translation termination, thus resulting in a ph more

Market studies Genetic Engineering

• Enzo Biochem, Inc. (ENZ) - Financial and Strategic SWOT Analysis Review

  Enzo Biochem, Inc. (ENZ) - Financial and Strategic SWOT Analysis Review Summary Enzo Biochem, Inc. (Enzo Biochem) is a life sciences and biotechnology company and is principally engaged in research, development, manufacturing and marketing of diagnostic and therapeutic products based on genetic en more

• BIOTON S.A. (BIO) - Financial and Strategic SWOT Analysis Review

  BIOTON S.A.(BIOTON) is a publicly held biotechnological company. It is engaged in the manufacture and distribution of pharmaceutical products with focus on insulin drugs. The company's products include lakcillus probiotic, recombinant human insulin for injections, oral hypoglycemic drugs, containin more

• Shanghai Fudan-zhangjiang Bio-Pharmaceutical Co.,Ltd. (8231) - Financial and Strategic SWOT Analysis Review

  Shanghai Fudan-zhangjiang Bio-Pharmaceutical Co., Ltd. (FDZJ) is a publically held company engaged in the research, development, manufacturing and commercialization of innovative therapeutics. The company focuses on development of therapeutics like genetic engineering drugs, photodynamic drugs, diag more

Websites Genetic Engineering