Topic fluorescent dyes

T-cells are the immune system’s security force. They seek out pathogens and rogue cells in the body and put them out of action. Their precursors are formed in the bone marrow and migrate from there into the thymus. Here, they mature and differentiate to perform a variety of tasks. Scientists at the more

Using a liquid laser, University of Michigan researchers have developed a better way to detect the slight genetic mutations that might predispose a person to a particular type of cancer or other diseases. Their results are published in Angewandte Chemie. This work could advance understanding of the more

Countless mice, rats and rabbits die every year in the name of science – and the situation is getting worse. While German laboratories used some 2.41 million animals for scientific research in 2005, by 2009 this number had grown to 2.79 million. One third were destined for fundamental biology resear more

The efficiency of antibody molecules is dependent on their stability. We introduce an assay setup where stability of antibodies is measured. Shaking stress is initiated by the FLUOstar Omega microplate reader and aggregation propensity of antibody candidates is measured in 96-well format more

MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18-25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA transcripts annotated. Although miRNAs represent a relatively abundant class of transcripts, their expression levels vary greatly among species and tissues. Less abundant miRNAs routinely fail detection using technologies such as cloning, northern hybridization and microarray analysis. Here, we present a real-time quantification method for accurate, sensitive, and economical detection of plant miRNAs based upon the stem-loop RT primer approach using an miRNA-specific primer coupled with a stem-loop specific Universal ProbeLibrary (UPL) probe. This approach represents a highly specific, sensitive, and easy-to-apply system to detect miRNAs. more

Abstract Multiparametric image analysis is highly preferable in revealing complicated biological processes. The multiplexing capability is demanding and is limited by spectral overlap of fluorescent dyes and the number of fluorescent channels available in an imaging platform, especially when both more

cDNA encoding VEGF and Ig-like extracellular domains 2-4 of VEGFR-1 (sFlt-12-4) were cloned into prokaryotic expression vectors pET32a and pQE60. Recombinant proteins were purifi ed (metal affi nity chromatography) and renatured. Chemiluminescent study for the interaction of recombinant VEGF and sFl more

Cellular membranes are heterogeneous in composition, and the prevailing theory holds that the structures responsible for this heterogeneity in vivo are small structures (10–200 nm), sterol- and sphingolipid-enriched, of different sizes, highly dynamic denominated rafts. Rafts are postulated to be pl more

Fluorotechnics Limited (Fluorotechnics) is a pharmaceutical and healthcare company engaged in developing consumables and small equipment for scientists to better measure biological processes using fluorescence. The company's products are used globally by its customers in research, quality control an more