Fluorescence Polarization

Companies Fluorescence Polarization

BioTek Instruments GmbH, Germany

BioTek is a leading developer and supplier of high-value microplate instrumentation and software solutions. Our instrumentation is used to accelerate the drug discovery process, to advance discoveries in genomics and proteomics, and to aid in the advancement of life science research. As part of the more

News Fluorescence Polarization

• New high-throughput screening technique makes probing puzzling proteins possible

  Understanding the tens of thousands of proteins that compose the human proteome has emerged as a key challenge of this century, and research efforts to date have already enabled major advances in drug discovery and understanding basic biology. But many potential avenues have been blocked by lack of more

• DiscoveRx Corporation Adds 7 New Kinase Assay Toolboxes to HitHunter Portfolio

  Fremont, Calif. DiscoveRx Corporation announced the availability of a new range of kinase assay toolboxes to its expanding line of assays for high throughput screening. The ready-to-use toolboxes cover a broad range of kinase targets. These toolboxes combine all the components required for customers more

White papers Fluorescence Polarization

• Predictor(TM) hERG Fluorescence Polarization Assay Kit performed on the PHERAstar/PHERAstar Plus
  

  The potential for cardiotoxic side-effects continues to challenge the development of small molecule based therapies. These intolerable side-effects are often precipitated by drug-induced long QT syndrome (LQT), which is often linked to blocking the human ether-a-go-go related gene (hERG) potassium channel. Although patch-clamp electrophysiology remains the gold standard for determining the interaction of compounds with the function of the hERG channel, radioligand displacement assays have proven to be a cost-effective initial alternative assay for hERG channel liability at early stages of compound development. more

• Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus
  

  Immunoglobulins or antibodies are proteins that are involved in the immune response. That makes them interesting drug targets. This application note shows the detection of the immunoglobulin IgG using an AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus. The AlphaLISA® technology utilizes oxygen to start a cascade of chemical reactions resulting in a luminescence signal at 615 nm. Sensitivity and Z´values obtained in different plate formats are presented. more

• Membrane Fluidity Measurements Using UV Fluorescence Polarization and the POLARstar Omega
  

  The POLARstar Omega microplate reader from BMG LABTECH was used to study membrane fluidity via fluorescence polarization in 96-well format. The method allows for the monitoring of large scale alterations in membrane fluidity (such as phase transitions), as well as more subtle changes in lipid dynamic. The change in polarization caused by dibucaine and propranolol were investigated in both liposomes and mitochondrial membranes more

Publications Fluorescence Polarization

• Prolonged stimuli alter the bacterial chemosensory clusters

  Summary The clustering of membrane‐bound receptors plays an essential role in various biological systems. A notable model system for studying this phenomenon is the bacterial chemosensory cluster that allows motile bacteria to navigate along chemical gradients in their environment. While the bas more

• A Novel Inhibitor of STAT3 Homodimerization Selectively Suppresses STAT3 Activity and Malignant Transformation

  STAT3–STAT3 dimerization, which involves reciprocal binding of the STAT3-SH2 domain to phosphorylated tyrosine-705 (Y-705), is required for STAT3 nuclear translocation, DNA binding, and transcriptional regulation of downstream target genes. Here, we describe a small molecule S3I-1757 capable of disr more

• Ni(II)‐NTA Modified Poly(ethylene imine) Glycopolymers: Physicochemical Properties and First In Vitro Study of Polyplexes Formed with HIV‐Derived Peptides

  Abstract Alternative delivery entities are desirable in immunotherapies in which polyplexes are widely formed by electrostatic interactions to induce cellular uptake processes for bioactive molecules. In our study, biocompatible Ni(II)‐nitrilo(triacetic acid)‐modified poly(ethylene imine)‐maltos more

Websites Fluorescence Polarization