Fluorescence Microscopy

Catalogues Fluorescence Microscopy

dianova secondary antibody catalog 2013 (German) - all products from Jackson ImmunoResearch

More than 3500 highly purified secondary antibodies & conjugates for all immunological staining methods for specific signals and low background more

News Fluorescence Microscopy

• Plate reader for drug discovery
  

  The trend towards automated high content assays, particularly for systems biology and drug discovery, has stimulated the development of automated fluorescence microscopy systems to image sample arrays. Today’s commercially available high content analysis (HCA) instruments predominantly employ fluore more

• Scientists find a brake that acts when cellular motors run too far

  An international team of scientists has shown how microtubules are interconnected into large networks. Like the poles of a tent, microtubules give shape to cells. By sliding microtubules along each other cells self-organize networks and relay forces that move chromosomes around during cell division. more

• Scientists copy the ways viruses deliver genes

  Scientists at the National Physical Laboratory (NPL) have mimicked the ways viruses infect human cells and deliver their genetic material. The research hopes to apply the approach to gene therapy – a therapeutic strategy to correct defective genes such as those that cause cancer. Gene therapy is sti more

White papers Fluorescence Microscopy

• Efficacy Comparison of Transfection Reagents in a Collecting Duct Cell Line
  

  The cortical collecting duct regulates ion and water homeostasis in the kidney. A cell line denoted M-1 has been established from the cortical collecting duct of a transgenic mouse that exhibits many functional properties of the original tissue. While this cell line is valuable for in vitro studies, the cells are not easily transfected. Frequently, adenovirus-mediated gene transfer is used. As adenovirus production requires several additional steps, we prefer chemical methods to achieve recombinant protein expression in M-1 cells. Previous attempts to transfect this cell line were unsuccessful with far less than 10% of transfectants, although different reagents were tried. Here we compare three commercially available transfection reagents in terms of the percentage of transfected cells and the suitability for using a reporter construct in M-1 cells. more

• Optimization of Transfection Conditions for Gene Expression, siRNA Knock-Down, and Live Cell Imaging Using FuGENE® HD Transfection Reagent
  

  The experimental method of introducing foreign mole­cules into cells is an important tool to facilitate studies in various biological fields. Transfection using lipophilic methods (liposomes or other cationic species) is a convenient means of introducing many different types of molecules (plasmid DNA, RNA, siRNA, and miRNA) into eukaryotic cells [1,2]. However, the optimal condition for incorporation depends on the type of transfection reagent and the cells being used, resulting in variable transfection efficiency and the extent of cell damage caused by transfection. more

• Structural and Chemical 3D Imaging with AFM and Raman.
  

  In the Life Sciences and Bio-medical Research, the characterization of chemical compounds within cells or tissues is one of the most important and difficult tasks for researchers to perform. The development of pharmaceuticals such as drug delivery coatings or capsules also requires as much information as possible about their chemical and structural compositions. For the fields of nanotechnology and materials science, a thorough knowledge of the samples' surface morphology on a sub-micron scale is essential more

Publications Fluorescence Microscopy

• Three-dimensional spheroids of adipose-derived mesenchymal stem cells are potent initiators of blood vessel formation in porous polyurethane scaffolds

  Publication date: June 2013 Source:Acta Biomaterialia, Volume 9, Issue 6 Author(s): M.W. Laschke , T.E. Schank , C. Scheuer , S. Kleer , S. Schuler , W. Metzger , D. Eglin , M. Alini , M.D. Menger Adipose-derived mesenchymal stem cells (adMSCs) exhibit a high angiogenic activity. Acco more

• Standard fluorescent imaging of live cells is highly genotoxic

  Abstract Fluorescence microscopy is commonly used for imaging live mammalian cells. Here, we describe studies aimed at revealing the potential genotoxic effects of standard fluorescence microscopy. To assess DNA damage, a high throughput platform for single cell gel electrophoresis is used (e.g. more

• Smart fluorescent proteins: Innovation for barrier‐free superresolution imaging in living cells

  During the past decade, several novel fluorescence microscopy techniques have emerged that achieve incredible spatial and temporal resolution beyond the diffraction limit. These microscopy techniques depend on altered optical setups, unique fluorescent probes, or post‐imaging analysis. Many of the more

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