1 day Sample preparation and quality control of nucleic acids

One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to asure reliable results. The course covers:

Overview of nucleic acid extraction methods
How to properly determine the concentration of purified nucleic acids
Storage of biological samples and purified nucleic acids
Quality control of purified material
Integrity of purified RNA
How to test for the presence of inhibitors.