PCR Basic Course

The PCR Basic Course is intended for participants without any previous knowledge in the field of PCR who plan to establish this technology in their labs. Therefore, besides the theoretical principles of PCR, we will intensely discuss the different reaction components. You will practize the set-up of several PCRs and you will perform the detection and data analysis on the basis of gel electrophoresis. Furthermore, the optimization of PCR conditions, primer design, and trouble shooting will also be a main part of the course. At the end of the seminar, you will be capable of performing a PCR assay and analyzing the data.

The theoretical part includes:

PCR basics
Optimization of PCR parameters
Basics of primer design
Overview of specific PCR applications, temperature gradient PCR, real time PCR, reverse transcription PCR
Trouble shooting
The practical part includes:

Set up of various PCR reactions, e.g. temperature gradient PCR
Detection of PCR products by means of gel electrophoresis
Analysis of results

Target Group
Technical and scientific staff members without any previous knowledge who are interested in using PCR-techniques in the laboratory.

PA4661 13.12. - 14.12.2012
Beginning of the course on the first day: 9:30 am
End of the course on the last day: approx. 4:00 pm

The fee for 2 days training amounts to 749,- € (plus VAT)