Cloning Strategies

In this one-and-a-half-day theory course, basic methods and strategies for the cloning of DNA fragments are explained in detail.

Topics covered include:

Cloning strategies: successful and efficient cloning
Vectors: possibilities and choice (standard cloning vectors, eukaryotic expression vectors, inducible systems, etc.)
Enzymes: isochizomeres, compatible ends, suitable standard enzymes, fill-in and blunt end, dephosphorylation, etc.
Restriction digest, modification, and preparation
Ligation: stoichiometry and discussion of different methods
Transformation: competent bacteria, generation, and efficiency control
Selection: resistance to antibiotics, blue-white-selection using ß- galactosidase genes, analysis and control of results
Clone analysis, mini and maxi preps
DNA quality and purity requirements: gel purification and isolation
Restriction mapping
Removal of stop codons
Tips and tricks for difficult cloning
Cloning examples
Trouble shooting

Target Group
Technicians and researchers with previous knowledge of molecular biology who want to get a comprehensive overview of cloning strategies and want to optimize their cloning protocols.

Dates
PA4591 01.10.2012 - 02.10.2012
Beginning of the course on the first day: 9:00 am
End of the course on the last day: approx. 1:00 pm

The fee for 1.5 days training amounts to 579,- € (plus VAT)