2 days Hands-on qPCR

This introductory course consists of a theoretical part and a practical part where participants get to do qPCR experiments by themselves under experienced supervision. The course contains:

Basic PCR theory

The theory of real-time PCR

Applications and possibilities of qPCR. Comparison of qPCR with regular PCR.

Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons...etc)

Different instrument platforms and their typical uses

Primer design

The problem of primer-dimer formation and how to minimize them

Probe design

Experimental design and optimization

Basic data handling and analysis

Day 2:

Introduction to quantification principles

Quantification strategies, uses and limitations

Calculations using different relative quantification methods

Strategies for normalization of qPCR data

In situ calibration for compensation of inhibition in samples

Absolute quantification


You find more information and register here: http://www.tataa.com/Courses/Courses.html