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One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to asure reliable results. The course covers:

Overview of nucleic acid extraction methods

How to properly determine the concentration of purified nucleic acids

Storage of biological samples and purified nucleic acids

Quality control of purified material

Integrity of purified RNA

How to test for the presence of inhibitors.

Event Date:   28-03-2012
Event Location:  
TATAA Biocenter
Vídenská 1083
14220 Prague
Czechia
Event Organiser:  
TATAA Biocenter
Odinsgatan 28
41103 Gothenburg
Tel.
0046317615700

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