To use all functions of this page, please activate cookies in your browser.
With an accout for my.bionity.com you can always see everything at a glance – and you can configure your own website and individual newsletter.
- My watch list
- My saved searches
- My saved topics
- My newsletter
TMB Liquid substrate for ELISA
Enzyme Linked Immuno Sorbent Assay (ELISA) is a widely used method for both research and diagnostic purposes. It is an assay where the reaction between the antigen and its antibody takes place linked to a solid phase. For visualization of the antigen-antibody reaction an enzyme and the related substrate offer a coloured reaction which can be measured spectrophotometrically. Different substrates are available to the enzyme conjugates used in ELISA. TMB (3, 3’, 5, 5’-tetramethylbenzidine) is the most commonly used substrate for the enzyme horseradish peroxidase (HRP) and the combination is one of the most acknowledged ELISA detection systems. ’’Mono-component TMB systems combine TMB, substrate buffer and hydrogen peroxide for safe, ready-to-use, one step products for direct use in your preferred ELISA format.
TMB yields a soluble blue end product read at 655 nm. The reaction may be stopped by 0.2 M sulphuric acid which offers a yellow end product read at 450 nm. Ready-to-use TMB substrates are available in different activity levels to match individual requirements. All types of ELISA TMB products are available also as prestained, colour coded products for maximum convenience.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "TMB_Liquid_substrate_for_ELISA". A list of authors is available in Wikipedia.|