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Sequencing by hybridization
Sequencing by Hybridization is a class of methods for determining the order in which nucleotides occur on a strand of DNA. Typically used for looking for small changes relative to a known DNA sequence. The binding of one strand of DNA to its complementary strand in the DNA double-helix (aka hybridization) is sensitive to even single-base mismatches when the hybrid region is short or is specialized mismatch detection proteins are present. This is exploited in a variety of ways, most notable via DNA chips or microarrays with thousands to billions of synthetic oligonucleotides found in a genome of interest plus many known variations or even all possible single-base variations.
This technology has largely been displaced by Sequencing-by-synthesis based methods.
Examples of commercial systems
Also see: Sequencing by ligation
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Sequencing_by_hybridization". A list of authors is available in Wikipedia.|