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Inverse polymerase chain reaction
Inverse polymerase chain reaction is a variant of polymerase chain reaction (PCR) when only one internal sequence is known. This is especially useful in identifying flanking sequences to various genomic inserts. Like other polymerase chain reaction processes, inverse polymerase chain reaction is used to amplify DNA samples, via the temperature-mediated enzyme DNA polymerase.
However, one of the key limitations of conventional polymerase chain reaction is that it requires primers complementary to the termini of the target DNA. This method allows PCR when only one internal sequence is known.
Inverse PCR involves a series of digestions and self-ligation before cutting by an endonuclease, resulting in known sequences at either end of the unknown sequence.
|This article is licensed under the GNU Free Documentation License. It uses material from the Wikipedia article "Inverse_polymerase_chain_reaction". A list of authors is available in Wikipedia.|