DNA Repair

News DNA Repair

• DNA damage: The dark side of respiration

  Adventitious changes in cellular DNA can endanger the whole organism, as they may lead to life-threatening illnesses like cancer. Researchers at Ludwig-Maximilians-Universitaet (LMU) in Munich now report how byproducts of respiration cause mispairing of subunits in the double helix. The DNA in our c more

• Excess estrogen in pregnancy can silence BRCA1 in daughters, increasing breast cancer risk

  Excess estrogen levels during pregnancy can disable, in their daughters, a powerful breast cancer tumor suppressor gene, say researchers at Georgetown Lombardi Comprehensive Cancer Center. They found the DNA repair gene BRCA1 to be silenced in one year-old girls exposed to a high hormonal fetal envi more

• A scanner for hereditary defects
  

  Our genetic material is constantly exposed to damage, which the body’s own proteins normally repair. One of these proteins works like a scanner, continually scouring the genetic material for signs of damage. Researchers from the Institute of Veterinary Pharmacology and Toxicology at the University o more

White papers DNA Repair

• Functional Analysis of Side Effects of Transfection Reagents in the Context of Bax-induced Apoptosis
  

  FuGENE® HD Transfection Reagent is well-suited for studying the impact of apoptosis-inducing proteins such as bax. Transfection with FuGENE® HD Transfection Reagent produced only minimal cytotoxic side effects while maintaining a high transfection efficiency. In contrast, cytotoxicity, and off-target effects of the other commercially available transfection reagents tested made it impossible to distinguish the impact of the bax protein from the impact of the transfection reagent itself more

• Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System
  

  The LightCycler® 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology. This proved to be a robust platform for performing MS-HRM experiments, allowing rapid and high-throughput studies of DNA methylation. more

Publications DNA Repair

• A genetic variant in ERCC2 is associated with gastric cancer prognosis in a Chinese population

  Endogenous and exogenous factors can induce DNA damage, leading to increased risk of cancer. Nucleotide excision repair (NER) is considered as the most versatile DNA repair pathway to deal with a variety of different DNA lesions. ERCC1 and ERCC2 are the two important proteins in NER pathway. In thi more

• The comet assay, DNA damage, DNA repair and cytotoxicity: hedgehogs are not always dead

  DNA damage is commonly measured at the level of individual cells using the so-called comet assay (single-cell gel electrophoresis). As the frequency of DNA breaks increases, so does the fraction of the DNA extending towards the anode, forming the comet tail. Comets with almost all DNA in the tail a more

• Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis

  Summary Proper transmission of DNA in dividing cells is crucial for the survival of eukaryotic organisms. During cell division, faithful segregation of replicated chromosomes requires their tight attachment, known as sister chromatid cohesion, until anaphase. Sister chromatid cohesion is establi more

Websites DNA Repair