DNA Methylation

News DNA Methylation

• A new European Consortium to boost our understanding of Inflammatory Bowel Diseases

  The Inflammatory Bowel Disease characterization by a multi-modal integrated biomarker study (IBD-Character) Consortium is a collaborative effort to advance our understanding of the inflammatory bowel diseases Ulcerative Colitis and Crohn's Disease and to increase diagnostic precision in detection of more

• Novel gene drives development of different types of ovarian cancer

  Researchers at Mayo Clinic Cancer Center have identified a novel gene that can contribute to a woman's susceptibility for developing ovarian cancer. Researchers identified the gene, called HNF1B, through large-scale analysis of more than 16,000 women with ovarian cancer and more than 26,000 healthy more

• GATC Biotech conducts research to detect cancer in women

  GATC Biotech has started research on early detection of breast and ovarian cancer with the goal of developing a test to identify tumor markers present in blood and link these markers to the presence of cancer. As a partner of the research project EpiFemCare, GATC Biotech will manage sample processin more

White papers DNA Methylation

• Frequent Switching of Polycomb Repressive Marks and DNA Hypermethylation in the PC3 Prostate Cancer Cell Line
  

  A key observation in these presented results was the magnitude of hypermethylation in PC3 cells, which occurred at almost all of the inactive CpG island genes. A second key observation was that when PRC marks were depleted, a PC3 hypermethylation was observed. One possible model that can explain these observations assumes that DNA methylation in PC3 cells sweeps all CpGs that are not physically protected (or "masked") (Figure 3). According to this "passive" methylation model, transcription initiation complexes at the TSS [RNA PolII and related chromatin marks, can serve as one masking mechanism (Figure 3a), and Polycomb repressive complexes can serve as another (Figure 3b). This view was also supported by recent data on DNA methylation patterns of mouse ESCs. It is important to note that even if Polycomb complexes mask loci from de novo DNA methylation, it is possible that PRC activity (or recruitment) is not masked by DNA methylation (Figure 3c). This may be the case in some of the de novo PRC targets we ob more

• Genome Sequencer 20 System: Breakthrough in a New Applications Age of Sequencing
  

  The Genome Sequencer 20 System is an ultra-high-throughput automated DNA sequencing system capable of carrying out and monitoring sequencing reactions in a massively parallel fashion. Since the Genome Sequencer 20 System provides a complete solution for ultra-high-throughput DNA sequencing, an individual researcher can for the first time prepare samples and sequencing reactions, generate sequence reads, and assemble genome sequence data within days. more

• Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System
  

  The LightCycler® 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology. This proved to be a robust platform for performing MS-HRM experiments, allowing rapid and high-throughput studies of DNA methylation. more

Publications DNA Methylation

• GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells [ARTICLES]

  piRNA (PIWI-interacting RNA) is a germ cell–specific small RNA in which biogenesis PIWI (P-element wimpy testis) family proteins play crucial roles. MILI (mouse Piwi-like), one of the three mouse PIWI family members, is indispensable for piRNA production, DNA methylation of retrotransposons presuma more

• Acquired Resistance to EGFR Inhibitors Is Associated with a Manifestation of Stem Cell–like Properties in Cancer Cells

  Acquired resistance to EGF receptor (EGFR) tyrosine kinase inhibitor (TKI) is a critical problem in the treatment of lung cancer. Although several mechanisms have been shown to be responsible for acquired resistance, all mechanisms have not been uncovered. In this study, we investigated the molecula more

• Childhood maltreatment is associated with distinct genomic and epigenetic profiles in posttraumatic stress disorder [Psychological and Cognitive Sciences]

  Childhood maltreatment is likely to influence fundamental biological processes and engrave long-lasting epigenetic marks, leading to adverse health outcomes in adulthood. We aimed to elucidate the impact of different early environment on disease-related genome-wide gene expression and DNA methylatio more

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