DNA Isolation Kits

Companies DNA Isolation Kits

dianova GmbH, Germany

We have been providing our research and routine customers premium antibodies, immunoassays and products for molecular biology since 1982. We take the time to carefully consider all your queries and are pleased to support you in selecting primary antibodies, secondary antibodies and detection system more

Products DNA Isolation Kits

foodproof® Salmonella: rapid, reliable, convenient

Extensive regulations govern the testing for Salmonella in food industry. Merck's foodproof® Salmonella detection kits comply with the latest ISO more

Efficient nucleic acid purification based on magnetic bead technology

The automated protocol is fast and easy to setup with all reagents present in prefilled cartridges and 12 samples can be completed in as little as 40 min more

New: Selective Isolation of Microbial DNA

A new technology now enables the enrichment of bacterial DNA from any clinical and non-clinical sample. Based on Molzym’s MolYsis™ technology a range more

White papers DNA Isolation Kits

• Quantitative Detection of Legionella pneumophila in Water Samples: Assay Transfer to the LightCycler® 480 Real-Time PCR System
  

  An assay to detect and quantify Legionella spp. in water samples was successfully transferred from a capillary-based LightCycler® Instrument to the novel multiwell plate-based LightCycler® 480 System. A previously described protocol was easily adapted by modifying the fluorescent label of the detection probe to match the filter set of the LightCycler® 480 Instrument. While reproducibility and analytical sensitivity of the assay were found to be comparable on both systems, the novel plate-based instrument allowed for higher sample throughput and reduced assay time per sample. more

• Experimental Workflow for Fast and Accurate Genotyping of Scrapie-resistant/sensitive Sheep
  

  Several scientific studies support the claim that natural scrapie is associated with polymorphisms at three codons within the sheep prion protein (PrP) gene. Genotyping of these codons (codon 136 [alanine, valine], 154 [arginine, histidine], and 171 [glutamine, arginine, histidine]) might enable breeding of sheep flocks with high resistance to scrapie. Fast and reliable high-throughput genotyping of sheep is a major issue, since scrapie is a serious problem in several European countries. To address this, an experimental procedure for DNA preparation and PCR-based detection of codon 136, 154, and 171 polymorphisms was established. more

• Isolation of DNA from Dried Blood Spot Using the High Pure PCR Template Preparation Kit
  

  DNA isolation from anti-coagulated whole blood for subsequent molecular analysis by PCR methods is well-established. In a standard laboratory setting, the blood is shipped in a blood collection vial containing the anticoagulant after phlebotomy. However, it might not always be possible to have blood samples shipped immediately, and for longer storage cooling is required. Freezing anticoagulated blood samples might interfere with the efficiency of DNA isolation afterwards. Thus, the collection and storage of whole blood samples on special filter paper has become more and more popular since it provides a simple and easy method to ship and to store the samples. Evaluation of established DNA isolation kits and procedures is required in order to recommend a protocol for DNA isolation from dried blood spots (DBS). Here, we report the assessment of the High Pure PCR Template Preparation Kit for DNA Isolation from DBS more

Publications DNA Isolation Kits

• A comparison of methods used to extract bacterial DNA from raw milk and raw milk cheese

  Abstract Aims:  In this study we compare seven different methods which have been designed or modified to extract total DNA from raw milk and raw milk cheese with a view to its subsequent use for the PCR of bacterial DNA. Materials and Results:  Seven extraction methods were employed to extract more

• An evaluation of commercial DNA extraction kits for the isolation of bacterial spore DNA from soil

  Abstract Aims:  To evaluate six commercial DNA extraction kits for their ability to isolate PCR‐quality DNA from Bacillus spores in various soil samples. Methods and Results:  Three soils were inoculated with various amounts of Bacillus cereus spores to simulate an outbreak or intentional rele more

• Bacterial community analysis of activated sludge: an evaluation of four commonly used DNA extraction methods

  The effectiveness of three commercially available direct DNA isolation kits (Mobio, Fast, Qiagen) and one published direct DNA extraction protocol (Bead) for extracting bacterial DNA from different types of activated sludge was investigated and mutually compared. The DNA quantity and purity were det more

Websites DNA Isolation Kits