Determination

Products Determination

Separate proteins faster

Proteins can be analyzed or purified up to 3x faster with pressure-resistant glass columns of KNAUER Bioline and specially reinforced agarose media more

News Determination

• New diagnostics company from Berlin

  Astra Biotech GmbH is a new company whose core focus is the development of diagnostics for the identification of an individual’s predisposition to disease. With this in mind, Astra Biotech GmbH have produced a significant portfolio of assays in areas such as allergies, genetically associated disorde more

• U of T Researchers Develop “Hi-Def” Copy Number Variation Decoder

  University of Toronto researchers have developed a new “high definition” computer program to analyze human DNA and more accurately detect genetic variants that affect individual traits like disease susceptibility and varying drug responses. A multi-disciplinary team combining computer science and b more

• Biotie Announces Restructuring Plan

  Biotie announced that it will implement a restructuring plan to focus its business exclusively on clinical development and will transfer all operations to its headquarters in Finland. The restructuring would result in significant personnel and cost reductions. These initiatives are designed to lever more

White papers Determination

• Real-Time PCR Quality Control for Gene Expression
  

  Quantitative real-time PCR (qPCR) has become the de facto standard for nucleic acid quantification. This achievement is due in large part to its sensitivity, specificity, accuracy, large dynamic range of linear quantification, and its speed. The qPCR technology has matured to a ready-to-use commonly available method in most molecular biology laboratories. Nevertheless, the reliability of the final quantification result depends heavily on all elements in the workflow, such as the quality of the input template (integrity and absence of inhibitors), the PCR assay (specificity, efficiency, limit of detection), and normalization strategy (validated reference genes). more

• Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System
  

  The LightCycler® 480 Instrument is a versatile platform for molecular studies. We assessed the performance of the instrument combined with the LightCycler® 480 High Resolution Melting Master mix for DNA methylation analysis using the methylation-sensitive high resolution melting (MS-HRM) methodology. This proved to be a robust platform for performing MS-HRM experiments, allowing rapid and high-throughput studies of DNA methylation. more

• Confocal Detection of NBT/BCIP In Situ Hybridization
  

  Confocal laser scanning reflection microscopy (CLSM) in combination with whole-mount in situ hybridization (WMISH) is a novel method to visualize gene expression patterns in three dimensions in whole embryos. The method uses conventional NBT/BCIP precipitation by alkaline phosphatase in WMISH samples and confocal detection by reflection microscopy. WMISH protocols can also be combined with fluorescent counterstainings such as DAPI, antibodies, GFP lines, and fluorescent in situ probes. Such double and triple labeling combined with confocal microscopy allows expression profiling at a cellular resolution in cell types or embryological structures of interest. Whole-mount reflection CLSM will thus greatly facilitate large-scale, cellular resolution expression profiling in vertebrate and invertebrate model organisms. more

Publications Determination

• Mucin-type fusion proteins with blood group A or B determinants on defined O-glycan core chains produced in glycoengineered Chinese hamster ovary cells and their use as immunoa ...

  Assays for quantification, and methods for removal, of anti-A and anti-B antibodies are the key for the success of ABO incompatible organ transplantation programs. In order to produce tools that can be used as substrates in tests for anti-A/anti-B quantification and specificity determination or as more

• Annotation and structural elucidation of bovine milk oligosaccharides and determination of novel fucosylated structures

  Bovine milk oligosaccharides (BMOs) are recognized by the dairy and food industries, as well as by infant formula manufacturers, as novel, high-potential bioactive food ingredients. Recent studies revealed that bovine milk contains complex oligosaccharides structurally related to those previously t more

• STL-based Analysis of TRAIL-induced Apoptosis Challenges the Notion of Type I/Type II Cell Line Classification

  by Szymon Stoma, Alexandre Donzé, François Bertaux, Oded Maler, Gregory Batt Extrinsic apoptosis is a programmed cell death triggered by external ligands, such as the TNF-related apoptosis inducing ligand (TRAIL). Depending on the cell line, the specific molecular mechanisms leading to cell death more

Market studies Determination

• Italy Pharmaceuticals and Healthcare Report Q2 2012

  BMI View: The pharmaceuticals and healthcare sector will be a target of further cost containment, particularly as National Health System (SSN) data for H111, which shows a decline in spending, highlights the success of previous cost-containment efforts. Headline Expenditure Projections ?? Pharmac more

• New Zealand Pharmaceuticals and Healthcare Report Q3 2012

  BMI View: New Zealand’s pharmaceutical regulatory regime continues to be of major concern for multinationals. For example, according to the Pharmaceutical Research and Manufacturers of America (PhRMA)’s Special 301 Submission for 2012 requested that New Zealand be placed on the Priority Watch List, more

• Slovenia Pharmaceuticals and Healthcare Report Q3 2012

  BMI View: Our view that Slovenia’s drug market is one of the most stable in the region remains in place despite the fact that - at the start of 2012 - the government published new measures to control public drug expenditure. The government’s methods are considerably less aggressive than those in oth more

Websites Determination