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• The Nobel Prize in Physiology or Medicine 2010 goes to Robert G. Edwards for the development of in vitro fertilization

  Robert Edwards is awarded the 2010 Nobel Prize for the development of human in vitro fertilization (IVF) therapy. His achievements have made it possible to treat infertility, a medical condition afflicting a large proportion of humanity including more than 10% of all couples worldwide. As early as more

• New approach to Alzheimer's therapy - researchers identify key target molecule

  Researchers from the German Centre for Neurodegenerative Diseases (DZNE) and the Ludwig-Maximilians-Universität (LMU) in Munich have shown that the ADAM10 protein can inhibit the formation of beta-amyloid, which is responsible for Alzheimer’s disease. ADAM10 acts like a pair of molecular scissors to more

• Probiodrug Further Expands Scientific Advisory Board by Appointing Prof Dr Dr hc Christian Haass

  Probiodrug AG announced the appointment of Prof Dr Dr hc Christian Haass to the Company's Scientific Advisory Board. In his work, Prof Haass focuses on the molecular and cellular mechanisms of Alzheimer's and Parkinson's disease. In Alzheimer's disease, he has elucidated the biochemistry and molecul more

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• Enzyme Kinetic Measurements for a Combinatorial Library of Inhibitors of Pseudomonas Elastase
  

  Pseudomonas elastase (LasB) is a key drug target in various chronic and intractable infections. A library of 160 potential, in-house synthesised, elastase inhibitors were screened using a fluorescence assay with the internally quenched substrate Abz-Ala-Gly-Leu-Ala-Nba. Enzymatic kinetic measurements were done on a FLUOstar OPTIMA and Km and Vmax values were calculated for each inhibitor using the MARS Data Analysis software more

• Characterizing Biotherapeutics with HPLC
  

  As demonstrated here, there are a broad range of HPLC applications used in development, production, validation and release of protein therapeutics for human use. Some of the applications mentioned above are mandatory when submitting a new biopharmaceutical to approval at the regulatory agencies. The introduction of the first so-called biosimilars in Europe further increased the demand for highly efficient analysis methods. The continuous development of new stationary phases for the common modes of biochromatography, such as size exclusion, ion exchange and hydrophilic interaction chromatography support biochemists in increasing lab productivity and establishing high-throughput HPLC analysis not only for small molecules but also for large biopolymers more

• RealTime ready RT-qPCR Assay Development and Qualification
  

  On the RealTime ready Configurator, we offer function tested human, mouse, and rat qPCR assays for gene expression quantification based on the unique Universal ProbeLibrary technology (UPL). The Universal ProbeLibrary is a set of short hydrolysis probes (8–9-mers) containing modified bases in order to stabilize the binding and thus ensure high flexibility together with good amplicon specificity more

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• Evolutionary Analysis of the Contact System Indicates that Kininogen Evolved Adaptively in Mammals and in Human Populations

  Activation of the contact system leads to the cleavage of kininogen by plasma kallikrein resulting in kinin release and in the initiation of the intrinsic pathway of coagulation. Proteolysis of kininogen also generates antimicrobial peptides (AMPs) and can be induced by diverse pathogens. Thus, the more

• Manuscript Title: Interaction of small RNA‐8105 and the Intron of IbMYB1 RNA Regulates IbMYB1 Family Genes through Secondary siRNAs and DNA Methylation after Wounding

  Summary Small RNAs (sRNAs) play important roles in plants encountering stress environments. However, limited research has been conducted on the sRNAs involved in plant wound responses. In the present study, a novel wounding‐induced sRNA, small RNA‐8105 (sRNA8105), was identified in sweet potato more

• The Specificity and Flexibility of L1 Reverse Transcription Priming at Imperfect T-Tracts

  by Clément Monot, Monika Kuciak, Sébastien Viollet, Ashfaq Ali Mir, Caroline Gabus, Jean-Luc Darlix, Gaël Cristofari L1 retrotransposons have a prominent role in reshaping mammalian genomes. To replicate, the L1 ribonucleoprotein particle (RNP) first uses its endonuclease (EN) to nick the genomic more

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