Topic World Chromatography

Chromatography enables us to separate, identify and thus understand complex substances. Whether in the food industry, pharmaceutical research or environmental analysis - chromatography opens up a treasure trove of information about the composition and quality of our samples. Discover the fascinating world of chromatography!

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ÄKTA pure – Flexible and Intuitive Protein Purification System ÄKTA pure – Flexible and Intuitive Protein Purification System

ÄKTA pure – Flexible and Intuitive Protein Purification System

ÄKTA™ lab-scale protein purification systems are designed for purification of biomolecules, providing speed, performance, and flexibility in research and process development. ÄKTA systems use intelligent UNICORN™ system control software to combine simplicity with power in protein purification from your lab bench today to process development and full-scale bioprocess production tomorrow. ÄKTA pure is a flexible and intuitive purification system for proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product in research applications. The ÄKTA pure system is available in two versions: ÄKTA pure 25, designed for a broad range of applications and purification tasks in a multi-user environment, and ÄKTA pure 150, well suited for routine larger-scale (tens of grams) preparative purification. Applications: Small scale preparative purification in research (ÄKTA pure 25) Larger-scale (tens of grams) purification (ÄKTA pure 150) Flow-rate ranges: 0.001–25 ml/min (ÄKTA pure 25) 0.01–150 ml/min (ÄKTA pure 150) Maximal operating pressures: 20 MPa (ÄKTA pure 25) 5 MPa (ÄKTA pure 150) Smart Solutions That Free up Your TimeÄKTA pure reduces hands-on time so that you can spend less time purifying proteins and more time focusing on your results. Easily switch between different users and applications. To get you up and running quickly, methods are easily created by dragging and dropping predefined templates in UNICORN 7. Purify with Confidence While You're Doing Other ThingsÄKTA pure allows unattended operations by efficiently automating your purification tasks. ÄKTA pure safeguards your samples and columns so that you can focus on other things. Programmable email notifications contact you when an alarm triggers.  Multi-Step Purification for Fast and Efficient Protein PurificationPurification of protein to sufficiently high yield and purity can be a time- and resource-consuming process. To address fast and efficient protein purification, ÄKTA pure can be set up in different ways to handle multi-step purification. What Our Customers Are Saying"The purification group has to deal with as much as about 1500 proteins/year, so that averages about 100 proteins/week.” “Because the system also can do as many as about seven samples per unit, it means you can actually get a lot more protein purified on the system at any given time." Dr. Lou Fabri, Director, Protein Technologies, Research, CSL Ltd., Melbourne, Australia ÄKTA club is a free online community for ÄKTA system users enabling easy and quick access to trustable information about ÄKTA systems and protein purification. Access to information about your ÄKTA system has never been so easy. Join us today.

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Topic world Gas chromatography

Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.

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Topic world Gas chromatography

Gas chromatography is an essential method in analytical chemistry for the separation and analysis of volatile compounds. Due to its high resolution and sensitivity, it has become firmly established in areas such as environmental analysis, food chemistry or forensic science. GC provides precise and reliable results and enables deep insights into the chemical composition of samples.

5+ products
4 brochures
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Chromatography explained

Affinity chromatography

Affinity chromatography Affinity chromatography is a chromatographic method of separating biochemical mixtures, based on a highly specific biologic interaction such as that between antigen and antibody , enzyme and substrate , or receptor and ligand . Affinity chromatography combines the size ...

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Chiral column chromatography

Chiral column chromatography Chiral column chromatography is a variant of column chromatography , where the stationary phase is chiral instead of achiral. The enantiomer s of the same compound then differ in affinity to the stationary phase, thus they exit the column at different times. The chir ...

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Chromatographic Separation Methods in Simple Terms

Separating mixtures of substances to analyze them qualitatively and quantitatively – these are the chromatographic methods you should know about ...

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Chromatography

Chromatography Chromatography (from Greek χρώμα:chroma, colour and γραφειν:"grafein" to write) is the collective term for a family of laboratory techniques for the separation of mixtures . It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates ...

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Column chromatography

Column chromatography Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The classical preparative chromatography column is a glass tube ...

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Fast protein liquid chromatography

Fast protein liquid chromatography Fast Protein Liquid Chromatography, usually referred to as FPLC, is a form of column chromatography used to separate or purify proteins from complex mixtures by using Ion Chromatography . It is very commonly used in biochemistry and enzymology . Columns used w ...

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Gas-liquid chromatography

Gas-liquid chromatography Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or an unreactive gas such as nitrogen , and the stationary phase is a microscopic layer of li ...

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Gel Permeation Chromatography

Gel Permeation Chromatography Gel permeation chromatography (GPC) is a separation technique based on hydrodynamic volume (size in solution). Molecules are separated from one another based on differences in molecular size. This technique is often used for polymer molecular weight determination. GP ...

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High performance liquid chromatography

High performance liquid chromatography High-performance liquid chromatography (HPLC) is a form of column chromatography used frequently in biochemistry and analytical chemistry . It is also sometimes referred to as high-pressure liquid chromatography. HPLC is used to separate components of a mixt ...

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Hydrophilic interaction liquid chromatography

Hydrophilic interaction liquid chromatography HILIC (HydrophILic Interaction Chromatography or Hydrophilic Interaction LIquid Chromatography) is a version of normal phase liquid chromatography a name suggested by Dr. Andrew Alpert in his 1990 paper on the subject (J. Chromatogr. 499 (1990) 177) whe ...

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Mixed-mode chromatography

Mixed-mode chromatography Mixed-mode chromatography is a type of chromatography . In liquid chromatography , several modes of separation are commonly applied to resolve a mixture of different molecules. Ion-exchange chromatography , normal phase chromatography , reversed phase chromatography , size ...

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Size exclusion chromatography

Size exclusion chromatography Size exclusion chromatography (SEC) is a chromatographic method in which particles are separated based on their size, or in more technical terms, their hydrodynamic volume . It is usually applied to large molecule s or macromolecular complexes such as proteins and ind ...

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Supercritical fluid chromatography

Supercritical fluid chromatography Supercritical Fluid Chromatography (SFC) is a robust and easy-to-use form of normal phase chromatography ideally suited to the analysis and purification of low to moderate molecular weight, thermally labile molecules. It is especially suited to the separation of ...

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The Separation Principle Behind Chromatography in Simple Terms

An overview of the physical and chemical basics and more ...

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Thin layer chromatography

Thin layer chromatography Thin layer chromatography (TLC) is a chromatography technique used to separate chemical compounds . It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel , aluminium oxide , or cellulose immobilised onto a flat, inert carrie ...

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