Biomol

Companies Biomol

Biomol GmbH, Germany

Biomol GmbH offers antibodies, enzymes, bioactive lipids, microarrays and reagents for biomedical research. Our focus is on cellular regulation and signal transduction. Together with our partners, we also provide a variety of specialized kits for drug discovery. more

Catalogues Biomol

Fat Cells, Immune Cells and Metabolic Syndrome

The Importance of the research into Metabolic Syndrome and related diseases is fascinating. Which methods and tools do experts use? more

News Biomol

• "Silent" fungus metabolism awakened for new natural products

  US scientists have re-awakened 'silent' metabolic pathways in fungi to reveal a new range of natural products. The research could provide not only a source of new drugs, but a way to "listen to what fungi are saying" to organisms around them. Fungi produce a wide variety of natural products, includ more

White papers Biomol

• Real-time and Dynamic Monitoring of Virus-mediated Cytopathogenicity
  

  To understand overall infectivity, virus progression and disease onset it is important to perform a detailed examination of cytopathic effects. Depending on the type of viruses and cells, different cytopathic effects might be observed dependent on the timepoint of infection (e.g., during the growth or stationary phase) and the amount of virus used. One of the most common methods for measuring lysogenic/lytic activity of viruses is the plaque test [1]. In this assay culture plates with confluent cells that are sensitive towards the specific virus type/strain are used. Lytic activity of a virus is detected by macroscopic analysis of plaques in a confluent cell layer after staining (e.g., with crystal violet). This technique is an end-point assay and it is well-suited to determine viral titers; however, it provides no information about the onset of cytopathic effects and kinetics of the viral replication. Moreover, infection with a number of viruses (e.g., attenuated mutants) leads to the formation of small and more

• Label-Free and Dynamic Monitoring of Cell-Based Assays
  

  The xCELLigence system from Roche Applied Science, originally invented by the US company ACEA Biosciences, allows for label-free and dynamic monitoring of cell-based assays. The xCELLigence system utilizes impedance readout to non-invasively quantify cellular status in real-time [1]. The cells are seeded in 96-well microtiter E-Plates with integrated microelectronic sensor arrays at the bottom of each well. more

Publications Biomol

• A unified NMR strategy for high-throughput determination of backbone fold of small proteins

  An efficient semi-automated strategy called PFBD (i.e. Protein Fold from Backbone Data only) has been presented for rapid backbone fold determination of small proteins. It makes use of NMR parameters involving backbone atoms only. These include chemical shifts, amide–amide NOEs and H-bonds. The back more

• Erratum to: Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion

  Erratum to: Immunostimulatory activity of ConBr: a focus on splenocyte proliferation and proliferative cytokine secretion Content Type Journal Article Category Erratum Pages 1-1 DOI 10.1007/s00441-011-1294-3 Authors Flávio de Oliveira Silva, Departamento de Morfologia e Fisiologia Anima more

• Chromophore-Assisted Light Inactivation of HaloTag Fusion Proteins Labeled with Eosin in Living Cells

  Chromophore-assisted light inactivation (CALI) is a potentially powerful tool for the acute disruption of a target protein inside living cells with high spatiotemporal resolution. This technology, however, has not been widely utilized, mainly because of the lack of an efficient chromophore as the ph more

Websites Biomol