Applied Biosystems

Companies Applied Biosystems

News Applied Biosystems

• Applied Biosystems Deutschland GmbH holds 95% of GENEART Shares

  The majority shareholder of GENEART AG, Regensburg, Applied Biosystems Deutschland GmbH, Darmstadt ("ABD"), directly holds more than 95% of all shares in GENEART AG as noticed by ABD. ABD informed the management board of GENEART AG about this fact by letter dated August/3/2010. By virtue of this le more

• GENEART AG and Applied Biosystems Deutschland GmbH intend to conclude a domination agreement

  GENEART AG and its majority shareholder, Applied Biosystems Deutschland GmbH, intend to conclude a domination agreement pursuant to Section 291 et seq. German Stock Corporation Act (Aktiengesetz) with GENEART AG as the dominated entity and Applied Biosystems Deutschland GmbH as the dominating entity more

• Management Board and Supervisory Board of GENEART Recommends Acceptance of Voluntary Public Offer

  The Management Board and the Supervisory Board of GENEART AG have announced their joint statement with respect to the voluntary public offer by Applied Biosystems Deutschland GmbH to the shareholders of GENEART AG. This joint statement is in reference to the offer document published by the Applied B more

White papers Applied Biosystems

• qPCR Validation of Candidates for Modulators of the MAPkinase Signaling Pathway
  

  With the completion of the human genome sequence, the detailed understanding of gene functions by analyzing the structure, biological activities, and dynamics of the encoded proteins has become the next challenge. The technology of RNA interference (RNAi), which allows the silencing of genes by introduction of small interfering RNA (siRNA), is recognized as an extremely powerful tool for systematic high-throughput functional genomic screening. more

• Fast and Sensitive Detection of Viral Targets in Quantitative RT-PCR Using the Universal One-Step qRT-PCR Master (ROX)
  

  For qRT-PCR assays it is a common procedure to perform two individual work steps. First, RNA is transcribed into cDNA, and then the target to be quantified is amplified separately. This two-step approach is believed to result in highest sensitivity of target detection. However, the sensitivity of nucleic acid amplification assays is determined by multiple factors and differs from target to target. In contrast, a one-step protocol in qRT-PCR benefits from short overall reaction times, shorter hands-on-times and low contamination risks. Here we show the highly sensitive quantitative detection of viral target RNA in real-time PCR to a copy number fewer than 10. more

• Rare Variants of IFIH1 Protect Against Type-1 Diabetes
  

  Nejentsev and co-workers have found that rare alleles of all associated IFIH1 polymorphisms consistently protect from type-1 diabetes, whereas IFIH1 alleles carried by the majority of the population predispose to the disease. This observation suggests that variants that disrupt IFIH1 function in the host antiviral response have been negatively selected rather than positively selected, because they confer protection from type-1 diabetes. Although the mechanisms by which IFIH1 polymorphisms contribute to type-1 diabetes pathogenesis remain to be explored, the authors noted that one of the protective variants is a non-sense mutation leading to a truncated 626-amino acid protein lacking the C-terminal helicase domain (Figure 1), whereas two other protective variants localize to the conserved splice donor sites and probably disrupt normal splicing of the IFIH1 transcript. This suggests that variants, which are predicted to reduce function of the IFIH1 protein, would decrease the risk of type-1 diabetes, whereas n more

Publications Applied Biosystems

• A rapid HPLC‐ESI‐MS/MS method for determination of β‐asarone, a potential anti‐epileptic agent, in plasma after oral administration of Acorus calamus extract to rats

  ABSTRACT β‐Asarone (BAS), a phenylpropanoid from Acorus calamus Linn., has shown biological effects in the management of cognitive impairment conditions such as Alzheimer's disease. The present paper describes a selective and sensitive liquid chromatography–tandem mass spectrometric method (HPLC more

• Novel approach for optimization of a ‘difficult’ peptide synthesis by utilizing quantitative reaction monitoring assays

  Three different synthetic strategies were tested to synthesize EGFRvIII peptide (1), which is a functional variant of the epidermal growth factor receptor, a protein that has been well validated as a target for cancer therapy. The initial synthesis was performed with Applied Biosystem (Carlsbad, C more

• Evaluation of the sensitivity of two recently developed STR multiplexes for the analysis of chimerism after haematopoietic stem cell transplantation

  Summary Forensic‐oriented kits analysing short tandem repeat (STR) polymorphisms are widely used to determine the proportions of donor and recipient cells after haematopoietic stem cell transplantation. The sensitivity of this technology is crucial for the early detection of relapse and, in conse more

Market studies Applied Biosystems

• Genomic Sequencing Technologies for Medicine: Market Prospects 2011-2021

  How will the genomic sequencing market develop this decade? Our new report shows you that industry and market's revenue potential from 2011 to 2021, discussing its prospects. This report covers technology to support genomics, with an emphasis on pharma R&D. We aim to save you time and aid your deci more

Websites Applied Biosystems