Automated monitoring and data collection contribute to significant time savings when using the xCELLigence System, with only a few minutes of extra setup time. For example, in a 96-well plate, 32 different conditions can be tested in triplicate; the wells need to be seeded with cells, monitored, and sampled. For both manual and xCELLigence System experiments, it takes approximately 1 hour to prepare the 32 conditions (including dilution series and mixing of components). Plating of the cells is more straightforward on the E-Plate, because a manual experiment involves manipulation of a number of vessels (e.g., 96 T25 flasks) to obtain relevant data for cell counting. more