PsbA (Rabbit, Anti-(Arabidopsis thaliana, Colobanthus quitensis kunt bartl, Hordeum vulgare, Marchantia polymorpha (liverwort), Nicotiana benthamiana, Panicum miliaceum, Panicum maximum, Pinus strobus, Maize (Zea mays), Physcomitrella patens, Chlamydomonas, Synechococcus pcc 7942, Anabaena pcc 7120, Prochlorococcus, Symbiodinium, Coscinodiscus wailesii))

KLH-conjugated synthetic peptide derived from available plant,algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana AtCg00020, Oryza sativa P0C434, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechococcus sp. P14660

; A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and 16kDa. D1 degradation is a complex set of events and the products observed can be influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559, shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA free peptide, indicating that all bands are indeed PsbA and its precursors or breakdown products. Competition assays were also performed with spinach and Chlamydomonas, confirming the identity of PsbA bands. Anti-PsbA antibodies will not detect D2 protein, as the peptide used to generate PsbA antibodies has no homology to the D2 sequence.