Adenine

News Adenine

• Carbon nanotubes show promise for high-speed genetic sequencing

  Faster sequencing of DNA holds enormous potential for biology and medicine, particularly for personalized diagnosis and customized treatment based on each individual's genomic makeup. At present however, sequencing technology remains cumbersome and cost prohibitive for most clinical applications, th more

• Models begin to unravel how single DNA strands combine

  Using computer simulations, a team of University of Wisconsin-Madison researchers has identified some of the pathways through which single complementary strands of DNA interact and combine to form the double helix. Understanding hybridization, the process through which single DNA strands combine more

• Scripps research team creates simple chemical system that mimics DNA

  A team of Scripps Research scientists has created a new analog to DNA that assembles and disassembles itself without the need for enzymes. Because the new system comprises components that might reasonably be expected in a primordial world, the new chemical system could answer questions about how lif more

White papers Adenine

• RealTime ready qPCR Assay Design and Configuration Portal Content
  

  The gene and transcript annotational content of the RealTime ready Configurator offers a combined view of three of the main gene annotation authorities. The positional information of the lab-tested RealTime ready assays regarding transcript or exon coverage is presented in a graphical overview and tabular format. Additional content provides a convenient and browsable entry point for target gene selection for cataloged as well as new assays that are developed on demand more

• Mutation Scanning Using High Resolution Melting or dHPLC: a Performance Comparison Study
  

  High Resolution Melting (HRM) is a novel and promising screening technique used for mutation analysis and detection. Its application prevents the need to sequence all the exons of a gene when looking for novel mutations. The method is therefore time- and cost-effective for the analysis of large genes comprising many exons. In the research project described here, we compared HRM on the LightCycler® 480 System with denaturing high-performance liquid chromatography (dHPLC), a more conventional method for mutation scanning. A WAVE 4500 HT System has been used for many years in our accredited human genetic laboratory for screening human samples by dHPLC, to identify unknown mutations that can then be characterized in detail by sequence analysis more

• Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer
  

  Nicotinamide Adenine Dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADP+) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) have been known to play vital roles in energy metabolism, antioxidation, and reductive biosynthesis. In recent years, these co-factors have been shown to be involved in other physiological functions as well, including aging, oxidative stress, intracellular calcium homeostasis, ROS production, cell death and gene expression. more

Publications Adenine

• Nucleobase‐caged peptide nucleic acids: PNA/PNA duplex destabilization and light‐triggered PNA/PNA recognition

  The 2‐(o‐nitrophenyl)‐propyl (NPP) group is used as caging group to mask the nucleobases adenine and cytosine in N‐(2‐aminoethyl)glycine peptide nucleic acids (aeg‐PNA). The adeninyl and cytosinyl nucleo amino acid building blocks Fmoc‐aNPP‐aeg‐OH and Fmoc‐cNPP‐aeg‐OH were synthesized and incorpor more

• Translating Enzymology into Metabolic Regulation: The Case of the 2- Oxoglutarate Dehydrogenase Multienzyme Complex

  Abstract: Enzymes are catalysts designed to function in the metabolic networks of biological systems. This review shows mechanisms underlying chemical contribution to the biological system performance and adaptations in permanently changing environment. The catalyst is exemplified by the 2-ox more

• Single-molecule chemical denaturation of riboswitches

  To date, single-molecule RNA science has been developed almost exclusively around the effect of metal ions as folding promoters and stabilizers of the RNA structure. Here, we introduce a novel strategy that combines single-molecule Förster resonance energy transfer (FRET) and chemical denaturation more

Websites Adenine