Enzymes, as biocatalysts, are a suitable, environmentally friendly alternative to heavy metal industrial catalysts. The “Green Chemistry” nature of enzymes as catalysts is based on a number of characteristics. Enzymes are biodegradable and typically produced through fermentation of renewable feedstocks or easily mass produced through recombinant technologies. Enzymes also catalyze chemical reactions under mild temperature (20°C-40˚C) and pH conditions (pH 5-8), and perform well in aqueous environments. The chemical, petroleum, agriculture, polymer, electronics and pharmaceutical industries rely on catalysis to optimize their chemical processes. It is estimated that at least 90% of chemicals produced rely to some extent on catalysis. In recent years researchers have increasingly focused their attention on green chemistry, including catalysis. Examples of enzyme-catalyzed industrial-scale organic reactions include: Hydrolysis Oxidation Reduction Addition – Elimination Halogenation and Dehalogenation Transesterification This webinar reviews case studies from pharma, academia and the military, showing how in situ mid-IR was used to monitor the enzyme-catalyzed reactions in real time to enable researchers to understand reaction mechanisms, and determine kinetic parameters and reduce costs.