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Hydration of protein–RNA recognition sites

01.11.2014 | Amita Barik; Ranjit Prasad Bahadur, Nucleic Acids Research, 2014

We investigate the role of water molecules in 89 protein–RNA complexes taken from the Protein Data Bank. Those with tRNA and single-stranded RNA are less hydrated than with duplex or ribosomal proteins. Protein–RNA interfaces are hydrated less than protein–DNA interfaces, but more than ...


Structure and mechanism of E. coli RNA 2',3'-cyclic phosphodiesterase [REPORT]

01.11.2014 | Barbara S. Remus; Agata Jacewicz; Stewart Shuman, RNA, 2014

2H (two-histidine) phosphoesterase enzymes are distributed widely in all domains of life and are implicated in diverse RNA and nucleotide transactions, including the transesterification and hydrolysis of cyclic phosphates. Here we report a biochemical and structural characterization of the ...


Insights from Stable Isotopic Tracers on Reproductive Allocation under Stress

01.11.2014 | C. L. Boggs; K. Niitepõld, Integrative and Comparative Biology, 2014

Fecundity is affected by changes in the nutritional and energetic environment, as a result of changes in acquisition, assimilation, or allocation of macro-nutrients and micro-nutrients. Stable isotopes of carbon and nitrogen offer a window into the processes underlying these changes. In insects ...


Spray-by-spray in situ cross-linking alginate hydrogels delivering a tea tree oil microemulsion

19.10.2014 | Author(s): O. Catanzano , M.C. Straccia , A. Miro , F. Ungaro , I. Romano , G. Mazzarella , G. Santagata , F. ..., European Journal of Pharmaceutical Science, 2014

Publication date: 23 January 2015 Source:European Journal of Pharmaceutical Sciences, Volume 66 Author(s): O. Catanzano , M.C. Straccia , A. Miro , F. Ungaro , I. Romano , G. Mazzarella , G. Santagata , F. Quaglia , P. Laurienzo , M. Malinconico In this paper we propose an in situ ...


Separation of Peanut Protein by Reverse Micelles: Optimization of the Forward Extraction

18.10.2014 | Xiaoyan Zhao, Jun Chen, Xiangyan Chen, Xianchang Wang, Yifeng Wang, Journal of Food Processing and Preservation, 2014

Abstract Bis(2‐ethylhexyl) sodium sulfosuccinate (AOT) reverse micelles were applied to extract the protein from peanut cake. In this study, the effect of temperature, amounts of peanut cake flour, particle size of peanut cake flour, pH, water content (W0), KCl concentration and time on the ...


Early physiological flood‐tolerance is followed by slow post‐flooding root recovery in the dryland riparian tree Eucalyptus camaldulensis subsp. refulgens

18.10.2014 | ARGUS R. E., COLMER T. D., GRIERSON P. F., Plant, Cell & Environment, 2014

Abstract We investigated physiological and morphological responses to flooding and recovery in Eucalyptus camaldulensis subsp. refulgens, a riparian tree species from a dryland region prone to intense episodic floods. Seedlings in soil flooded for 88 days produced extensive adventitious roots, ...


Quality changes during the frozen storage of the crustacean lobster krill (Munida spp.)

17.10.2014 | Bibiana García‐Soto, José M. Miranda, Jorge Barros‐Velázquez, Santiago P. Aubourg, European Journal of Lipid Science and Technology, 2014

This study focused on the quality changes of an undervalorised and underutilised crustacean, lobster krill (Munida spp.) during its frozen storage (10 months at ‐18°C). Fresh lobster krill specimens were previously soaked in aq. 0.75% and 0.25% sodium metabisulphite (SMB) solutions (0.75‐SMB ...


Synthesis and antitumor activity of natural compound Aloe emodin derivatives

17.10.2014 | N.R. Thimmegowda, Chanmi Park, B. Shwetha, Krisada Sakchaisri, Kangdong Liu, Joonsung Hwang, Sangku Lee, Sook Jung J ..., Chemical Biology & Drug Design, 2014

Abstract In the present study we have synthesized novel water soluble derivatives of natural compound aloe emodin 4(a‐j) by coupling with various amino acid esters and substituted aromatic amines, in an attempt to improve the anticancer activity and to explore the structure‐activity ...


Potent Effects of the Total Saponins from Dioscorea nipponica Makino Against Streptozotocin‐Induced Type 2 Diabetes Mellitus in Rats

17.10.2014 | Hao Yu, Lingli Zheng, Lina Xu, Lianhong Yin, Yuan Lin, Hua Li, Kexin Liu, Jinyong Peng, Phytotherapy Research, 2014

Abstract The aim of the present paper was to investigate the effects and possible mechanisms of the total saponins from Dioscorea nipponica Makino (TSDN) against type 2 diabetes mellitus. Streptozotocin (STZ) with high‐fat diet induced type 2 diabetes mellitus (T2DM) rats were treated with ...


Soil Temperature Determines the Reaction of Olive Cultivars to Verticillium dahliae Pathotypes

17.10.2014 | Rocío Calderón et al., PLoS ONE, 2014

by Rocío Calderón, Carlos Lucena, José L. Trapero-Casas, Pablo J. Zarco-Tejada, Juan A. Navas-Cortés Background Development of Verticillium wilt in olive, caused by the soil-borne fungus Verticillium dahliae, can be influenced by biotic and environmental factors. In this study we modeled i) the ...


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