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754 Aktuelle Fachpublikationen zum Thema Addition

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Probing the elastic limit of DNA bending

01.12.2014 | Tung T. Le; Harold D. Kim, Nucleic Acids Research, 2014

Sharp bending of double-stranded DNA (dsDNA) plays an essential role in genome structure and function. However, the elastic limit of dsDNA bending remains controversial. Here, we measured the opening rates of small dsDNA loops with contour lengths ranging between 40 and 200 bp using ...

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Negative reinforcement impairs overnight memory consolidation [BRIEF COMMUNICATIONS]

01.11.2014 | Andrew W. Stamm; Nam D. Nguyen; Benjamin J. Seicol; Abigail Fagan; Angela Oh; Michael Drumm; Maureen Lundt; Robert S ..., Learning & Memory, 2014

Post-learning sleep is beneficial for human memory. However, it may be that not all memories benefit equally from sleep. Here, we manipulated a spatial learning task using monetary reward and performance feedback, asking whether enhancing the salience of the task would augment overnight memory ...

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Transcriptional adaptation of Shigella flexneri during adherence to epithelial cells

07.10.2014 | Zhiwei Ni, Lingyan Jiang, Lu Feng, Lei Wang, Bin Liu, Journal of Basic Microbiology, 2014

Shigella adhesion to host cells is a transitional stage from an extracellular to an intracellular environment. However, the dynamic adaptations of Shigella during adhesion are poorly understood. To address this, we performed the first transcriptome analysis of Shigella flexneri 2457T during ...

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Compositional and immunobiological analyses of extracellular vesicles released by Candida albicans

07.10.2014 | Vargas G., Rocha J. D. B., Oliveira D. L., Albuquerque P. C., Frases S., Santos S. S., Nosanchuk J. D., Gomes A. M. ..., Cellular Microbiology, 2014

Summary The release of extracellular vesicles (EV) by fungal organisms is considered an alternative transport mechanism to trans‐cell wall passage of macromolecules. Previous studies have revealed the presence of EV in culture supernatants from fungal pathogens, such as Cryptococcus ...

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Effect of Osmolytes on the Binding of EGR1 Transcription Factor to DNA

01.10.2014 | David C. Mikles, Vikas Bhat, Brett J. Schuchardt, Caleb B. McDonald, Amjad Farooq, Biopolymers, 2014

ABSTRACT Osmolytes play a key role in maintaining protein stability and mediating macromolecular interactions within the intracellular environment of the cell. Herein, we show that osmolytes such as glycerol, sucrose and PEG400 mitigate the binding of EGR1 transcription factor to DNA in a ...

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Comparative Analysis of MAMP-induced Calcium Influx in Arabidopsis Seedlings and Protoplasts

01.10.2014 | Jens Maintz; Meltem Cavdar; Janina Tamborski; Mark Kwaaitaal; Rik Huisman; Christian Meesters; Erich Kombrink; Ralph ..., Plant and Cell Physiology, 2014

Rapid transient elevation of cytoplasmic calcium (Ca2+) levels in plant cells is an early signaling event triggered by many environmental cues including abiotic and biotic stresses. Cellular Ca2+ levels and their alterations can be monitored by genetically encoded reporter systems such as the ...

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Rice ORMDL Controls Sphingolipid Homeostasis Affecting Fertility Resulting from Abnormal Pollen Development

05.09.2014 | Chutharat Chueasiri et al., PLoS ONE, 2014

by Chutharat Chueasiri, Ketsuwan Chunthong, Keasinee Pitnjam, Sriprapai Chakhonkaen, Numphet Sangarwut, Kanidta Sangsawang, Malinee Suksangpanomrung, Louise V. Michaelson, Johnathan A. Napier, Amorntip Muangprom The orosomucoids (ORM) are ER-resisdent polypeptides encoded by ORM and ORMDL ...

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Different Dose-Dependent Mechanisms Are Involved in Early Cyclosporine A-Induced Cholestatic Effects in HepaRG Cells

01.09.2014 | Ahmad Sharanek; Pamela Bachour-El Azzi; Houssein Al-Attrache; Camille C. Savary; Lydie Humbert; Dominique Rainteau; ..., Toxicological Sciences, 2014

Mechanisms involved in drug-induced cholestasis in humans remain poorly understood. Although cyclosporine A (CsA) and tacrolimus (FK506) share similar immunosuppressive properties, only CsA is known to cause dose-dependent cholestasis. Here, we have investigated the mechanisms implicated in early ...

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PLAGL2 regulates actin cytoskeletal architecture and cell migration

01.09.2014 | Ryuichiro Sekiya; Masao Maeda; Hong Yuan; Eri Asano; Toshinori Hyodo; Hitoki Hasegawa; Satoko Ito; Kiyosumi Shibata; ..., Carcinogenesis , 2014

Pleomorphic adenoma gene like-2 (PLAGL2), a member of the PLAG gene family, is a C2H2 zinc finger transcriptional factor that is involved in cellular transformation and apoptosis. In this report, we show that PLAGL2 is associated with the organization of stress fibers and with small guanosine ...

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Deficiency of {alpha}-glucosidase I alters glycoprotein glycosylation and lifespan in Caenorhabditis elegans

01.10.2013 | Toshihiko Katoh; Juri Takase; Yasushi Tani; Ryuta Amamoto; Naofumi Aoshima; Michael Tiemeyer; Kenji Yamamoto; Hisash ..., Glycobiology , 2013

Endoplasmic reticulum (ER) α-glucosidase I is an enzyme that trims the distal α1,2-linked glucose (Glc) residue from the Glc3Man9GlcNAc2 oligosaccharide following its addition to nascent glycoproteins in the initial step of processing. This reaction is critical to the subsequent processing of ...

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