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Heparosan-glucuronate 5-epimerase: Molecular cloning and characterization of a novel enzyme

01.07.2015 | Hideo Mochizuki; Kiwamu Yamagishi; Kiyoshi Suzuki; Yeong Shik Kim; Koji Kimata, Glycobiology , 2015

Iduronic acid (IdoA) is a critical component of heparan sulfate in its interaction with functional proteins. Heparosan-N-sulfate-glucuronate 5-epimerase (HNSG-5epi) converts d-glucuronic acid (GlcA) residues in N-sulfated heparosan (NS-heparosan), as an intermediate in heparan sulfate ...

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Matriglycan: a novel polysaccharide that links dystroglycan to the basement membrane

01.07.2015 | Takako Yoshida-Moriguchi; Kevin P Campbell, Glycobiology , 2015

Associations between cells and the basement membrane are critical for a variety of biological events including cell proliferation, cell migration, cell differentiation and the maintenance of tissue integrity. Dystroglycan is a highly glycosylated basement membrane receptor, and is involved in ...

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Alteration of sperm protein profile induced by cigarette smoking

01.07.2015 | Xiaohui Chen; Wangjie Xu; Maohua Miao; Zijue Zhu; Jingbo Dai; Zhong Chen; Peng Fang; Junqing Wu; Dongsheng Nie; Lian ..., Acta Biochimica et Biophysica Sinica, 2015

Cigarette smoking is associated with lower semen quality, but how cigarette smoking changes the semen quality remains unclear. The aim of this study was to screen the differentially expressed proteins in the sperm of mice with daily exposure to cigarette smoke. The 2D gel electrophoresis (2DE) ...

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L-GRAAL: Lagrangian graphlet-based network aligner

01.07.2015 | Noël Malod-Dognin; Nataša Pržulj, Bioinformatics, 2015

Motivation: Discovering and understanding patterns in networks of protein–protein interactions (PPIs) is a central problem in systems biology. Alignments between these networks aid functional understanding as they uncover important information, such as evolutionary conserved pathways, protein ...

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Electrophysiological, hemodynamic and mitochondrial alterations induced by levobupivacaine during myocardial ischemia in a pig model. Protection by lipid emulsions?

27.06.2015 | Zahida Mamou, Jacques Descotes, Philippe Chevalier, Bernard Bui‐xuan, Caroline Romestaing, Quadiri Timour, Fundamental & Clinical Pharmacology, 2015

Abstract Accidental intravascular or high‐dose injection of local anesthetics (LA) can result in serious, potentially life‐threatening complications. Indeed, adequate supportive measures and the administration of lipid emulsions are required in such complications. The study's objectives were ...

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Advanced Evaluation Strategies for Protein‐Imprinted Polymer Nanobeads

26.06.2015 | Bettina Pluhar, Boris Mizaikoff, Macromolecular Bioscience, 2015

Molecularly imprinted polymers (MIPs) are synthetic affinity matrices capable of selective binding a specific target molecule. A strategy for competitive selectivity studies is developed providing information on the selective binding properties of MIPs in complex matrices. Batch rebinding ...

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DLK1 is a novel inflammatory inhibitor which interferes with NOTCH1 signaling in TLR‐activated murine macrophages

26.06.2015 | Maria J. González, Almudena Ruiz‐García, Eva M. Monsalve, Ricardo Sánchez‐Prieto, Jorge Laborda, María J.M. Díaz‐Gue ..., European Journal of Immunology, 2015

Delta‐like protein 1 (DLK1) is a non‐canonical ligand that inhibits NOTCH1 receptor activity and regulates multiple differentiation processes. In macrophages, NOTCH signaling increases TLR‐induced expression of key pro‐inflammatory mediators. We have investigated the role of DLK1 in macrophage ...

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Curcumin Improves Amyloid β-Peptide (1-42) Induced Spatial Memory Deficits through BDNF-ERK Signaling Pathway

26.06.2015 | Lu Zhang et al., PLoS ONE, 2015

by Lu Zhang, Yu Fang, Yuming Xu, Yajun Lian, Nanchang Xie, Tianwen Wu, Haifeng Zhang, Limin Sun, Ruifang Zhang, Zhenhua Wang Curcumin, the most active component of turmeric, has various beneficial properties, such as antioxidant, anti-inflammatory, and antitumor effects. Previous studies have ...

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Viruses, Vol. 7, Pages 3483-3499: Pan-Genome Analysis of Brazilian Lineage A Amoebal Mimiviruses

26.06.2015 | Assis, Felipe L.; Bajrai, Leena ; Abrahao, Jonatas S.; Kroon, Erna G.; Dornas, Fabio P.; Andrade, Kétyllen R.; Borat ..., Viruses, 2015

Since the recent discovery of Samba virus, the first representative of the family Mimiviridae from Brazil, prospecting for mimiviruses has been conducted in different environmental conditions in Brazil. Recently, we isolated using Acanthamoeba sp. three new mimiviruses, all of lineage A of ...

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Spatio-temporal Dynamics and Mechanisms of Stress Granule Assembly

26.06.2015 | Daisuke Ohshima et al., PLoS Computational Biology, 2015

by Daisuke Ohshima, Kyoko Arimoto-Matsuzaki, Taichiro Tomida, Mutsuhiro Takekawa, Kazuhisa Ichikawa Stress granules (SGs) are non-membranous cytoplasmic aggregates of mRNAs and related proteins, assembled in response to environmental stresses such as heat shock, hypoxia, endoplasmic reticulum ...

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