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21.819 Aktuelle Fachpublikationen zum Thema Addition

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O-Glycan analysis of cellobiohydrolase I from Neurospora crassa

01.06.2016 | Shu-Lun Tang; Patricia Bubner; Stefan Bauer; Chris R Somerville, Glycobiology , 2016

We describe here the composition of the O-linked glycans on the Neurospora crassa cellobiohydrolase I (CBHI), which accounts for approximately 40% of the protein secreted by cells growing in the presence of cellulose. CBHI is O-glycosylated with six types of linear, and three types of branched, ...

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Hypoxia induces NO-dependent release of heparan sulfate in fibroblasts from the Alzheimer mouse Tg2576 by activation of nitrite reduction

01.06.2016 | Fang Cheng; Erika Bourseau-Guilmain; Mattias Belting; Lars-Åke Fransson; Katrin Mani, Glycobiology , 2016

There is a functional relationship between the heparan sulfate proteoglycan glypican-1 and the amyloid precursor protein (APP) of Alzheimer disease. In wild-type mouse embryonic fibroblasts, expression and processing of the APP is required for endosome-to-nucleus translocation of ...

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Is Spontaneous Translocation of Polar Lipids Between Cellular Organelles Negligible?

04.05.2016 | Pentti Somerharju, Lipid Insights, 2016

In most reviews addressing intracellular lipid trafficking, spontaneous diffusion of lipid monomers between the cellular organelles is considered biologically irrelevant because it is thought to be far too slow to significantly contribute to organelle biogenesis. This view is based on ...

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Tannic Acid Inhibits Proliferation, Migration, Invasion of Prostate Cancer and Modulates Drug Metabolizing and Antioxidant Enzymes

04.05.2016 | Karakurt, Serdar; Adali, Orhan, Anti-Cancer Agents in Medicinal Chemistry , 2016

Abstract: The aim of this study was to investigate the effects of plant phenolic compound tannic acid (TA) on proliferative, metastatic, invasive properties of prostate cancer (PCa) cells; PC-3 and LNCaP, as well as drug metabolizing and antioxidant enzymes. Characterization of TA was done ...

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Synthesis and Pharmacological Evaluation of Maleopimaric N-arylimides: Identification of Novel Proapoptotic Agents

04.05.2016 | Yao, Guiyang; Ye, Manyi; Zhu, Yongtao; Liao, Zhixin; Wang, Hengshan, Anti-Cancer Agents in Medicinal Chemistry , 2016

Abstract: Several N-aryl maleopimaric acid diimides (3a-3d, 4a-4g) were synthesized and evaluated their topoisomerase I inhibitory activities along with cytotoxicities against NCI, MGC-803, Bel-7404 and Hct-116 cell lines. The pharmacological dates revealed that most of structure analogs ...

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Structural and enzymatic analyses of a glucosyltransferase Alr3699/HepE involved in Anabaena heterocyst envelop polysaccharide biosynthesis

01.05.2016 | Xue-Ping Wang; Yong-Liang Jiang; Ya-Nan Dai; Wang Cheng; Yuxing Chen; Cong-Zhao Zhou, Glycobiology , 2016

Formation of the heterocyst envelope polysaccharide (HEP) is a key process for cyanobacterial heterocyst differentiation. The maturation of HEP in Anabaena sp. strain PCC 7120 is controlled by a gene cluster termed HEP island in addition to an operon alr3698-alr3699, which encodes two putative ...

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Structure and biological activity of a fucosylated chondroitin sulfate from the sea cucumber Cucumaria japonica

01.05.2016 | Nadezhda E Ustyuzhanina; Maria I Bilan; Andrey S Dmitrenok; Alexander S Shashkov; Mikhail I Kusaykin; Valentin A Sto ..., Glycobiology , 2016

A fucosylated chondroitin sulfate (FCS) was isolated from the body wall of Pacific sea cucumber Cucumaria japonica by extraction in the presence of papain followed by Cetavlon precipitation and anion-exchange chromatography. FCS was shown to contain d-GalNAc, d-GlcA, l-Fuc and sulfate in molar ...

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Urinary {beta}-galactosidase stimulates Ca2+ transport by stabilizing TRPV5 at the plasma membrane

01.05.2016 | Elizabeth H P Leunissen; Maxime G Blanchard; Fareeba Sheedfar; Marla Lavrijsen; Jenny van der Wijst; René J M Bindel ..., Glycobiology , 2016

Transcellular Ca2+ transport in the late distal convoluted tubule and connecting tubule (DCT2/CNT) of the kidney is a finely controlled process mediated by the transient receptor potential vanilloid type 5 (TRPV5) channel. A complex-type-N-glycan bound at the extracellular residue Asn358 of TRPV5 ...

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Biochemical characterization of an {alpha}1,2-colitosyltransferase from Escherichia coli O55:H7

01.05.2016 | Zhigang Wu; Guohui Zhao; Tiehai Li; Jingyao Qu; Wanyi Guan; Jiajia Wang; Cheng Ma; Xu Li; Wei Zhao; Peng G Wang; Lei Li, Glycobiology , 2016

Colitose, also known as 3,6-dideoxy-l-galactose or 3-deoxy-l-fucose, is one of only five naturally occurring 3,6-dideoxyhexoses. Colitose was found in lipopolysaccharide of a number of infectious bacteria, including Escherichia coli O55 & O111 and Vibrio cholera O22 & O139. To date, no ...

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The valine and lysine residues in the conserved FxVTxK motif are important for the function of phylogenetically distant plant cellulose synthases

01.05.2016 | Erin Slabaugh; Tess Scavuzzo-Duggan; Arielle Chaves; Liza Wilson; Carmen Wilson; Jonathan K Davis; Daniel J Cosgrove ..., Glycobiology , 2016

Cellulose synthases (CESAs) synthesize the β-1,4-glucan chains that coalesce to form cellulose microfibrils in plant cell walls. In addition to a large cytosolic (catalytic) domain, CESAs have eight predicted transmembrane helices (TMHs). However, analogous to the structure of BcsA, a bacterial ...

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