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Deficiency of {alpha}-glucosidase I alters glycoprotein glycosylation and lifespan in Caenorhabditis elegans

01.10.2013 | Toshihiko Katoh; Juri Takase; Yasushi Tani; Ryuta Amamoto; Naofumi Aoshima; Michael Tiemeyer; Kenji Yamamoto; Hisash ..., Glycobiology , 2013

Endoplasmic reticulum (ER) α-glucosidase I is an enzyme that trims the distal α1,2-linked glucose (Glc) residue from the Glc3Man9GlcNAc2 oligosaccharide following its addition to nascent glycoproteins in the initial step of processing. This reaction is critical to the subsequent processing of ...


Fucosyltransferase VII improves the function of selectin ligands on cord blood hematopoietic stem cells

01.10.2013 | Xiang Wan; Hidetaka Sato; Hiromasa Miyaji; J Michael McDaniel; Yuesi Wang; Etsuji Kaneko; BreeAnna Gibson; Padmaja M ..., Glycobiology , 2013

Selectins and their carbohydrate ligands mediate the homing of hematopoietic stem/progenitor cells (HSPCs) to the bone marrow. We have previously shown that ex vivo fucosylation of selectin ligands on HSPCs by α1,3 fucosyltransferase VI (FUT6) leads to improved human cord blood (CB)-HSPC ...


Drosophila TDP-43 dysfunction in glia and muscle cells cause cytological and behavioural phenotypes that characterize ALS and FTLD

01.10.2013 | Danielle C. Diaper; Yoshitsugu Adachi; Luke Lazarou; Max Greenstein; Fabio A. Simoes; Angelique Di Domenico; Daniel ..., Human Molecular Genetics, 2013

Amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD) are neurodegenerative disorders that are characterized by cytoplasmic aggregates and nuclear clearance of TAR DNA-binding protein 43 (TDP-43). Studies in Drosophila, zebrafish and mouse demonstrate that the neuronal ...


Tissue-to-blood distribution coefficients in the rat: Utility for estimation of the volume of distribution in man

16.09.2013 | Author(s): Paulo Paixão , Natália Aniceto , Luís F. Gouveia , José A.G. Morais, European Journal of Pharmaceutical Science, 2013

Publication date: 20 November 2013 Source:European Journal of Pharmaceutical Sciences, Volume 50, Issues 3–4 Author(s): Paulo Paixão , Natália Aniceto , Luís F. Gouveia , José A.G. Morais A compilation of rat tissue-to-blood partition coefficient data obtained both in vitro and in vivo ...


Dual-crosslinked oxidized, methacrylated alginate/PEG hydrogels for bioadhesive applications

16.09.2013 | Author(s): Oju Jeon , Julia E. Samorezov , Eben Alsberg, Acta Biomaterialia, 2013

Publication date: Available online 12 September 2013 Source:Acta Biomaterialia Author(s): Oju Jeon , Julia E. Samorezov , Eben Alsberg A degradable, cytocompatible bioadhesive can facilitate surgical procedures and minimize patient pain and postsurgical complications. In this study, a ...


Effect of addition of hyaluronic acids on osteoconductivity and biodegradability of synthetic octacalcium phosphate

16.09.2013 | Author(s): Kentaro Suzuki , Takahisa Anada , Tatsuya Miyazaki , Naohisa Miyatake , Yoshitomo Honda , Koshi N. ..., Acta Biomaterialia, 2013

Publication date: Available online 12 September 2013 Source:Acta Biomaterialia Author(s): Kentaro Suzuki , Takahisa Anada , Tatsuya Miyazaki , Naohisa Miyatake , Yoshitomo Honda , Koshi N. Kishimoto , Masami Hosaka , Hideki Imaizumi , Eiji Itoi , Osamu Suzuki The present study was ...


Silencing of the Drosophila ortholog of SOX5 in heart leads to cardiac dysfunction as detected by optical coherence tomography

15.09.2013 | Airong Li; Osman O. Ahsen; Jonathan J. Liu; Chuang Du; Mary L. McKee; Yan Yang; Wilma Wasco; Christopher H. Newton-C ..., Human Molecular Genetics, 2013

The SRY-related HMG-box 5 (SOX5) gene encodes a member of the SOX family of transcription factors. Recently, genome-wide association studies have implicated SOX5 as a candidate gene for susceptibility to four cardiac-related endophenotypes: higher resting heart rate (HR), the electrocardiographic ...


An integrated toolkit for accurate prediction and analysis of cis-regulatory motifs at a genome scale

15.09.2013 | Qin Ma; Bingqiang Liu; Chuan Zhou; Yanbin Yin; Guojun Li; Ying Xu, Bioinformatics, 2013

Motivation: We present an integrated toolkit, BoBro2.0, for prediction and analysis of cis-regulatory motifs. This toolkit can (i) reliably identify statistically significant cis-regulatory motifs at a genome scale; (ii) accurately scan for all motif instances of a query motif in specified ...


pyRMSD: a Python package for efficient pairwise RMSD matrix calculation and handling

15.09.2013 | Víctor A. Gil; Víctor Guallar, Bioinformatics, 2013

Summary: We introduce pyRMSD, an open source standalone Python package that aims at offering an integrative and efficient way of performing Root Mean Square Deviation (RMSD)-related calculations of large sets of structures. It is specially tuned to do fast collective RMSD calculations, as ...


Effect of TAT‐obestatin on proliferation, differentiation, apoptosis and lipolysis in 3T3‐L1 preadipocytes

14.09.2013 | Guangcai Ren, Zuyong He, Peiqing Cong, Jingwei Yu, Yufeng Qin, Yaosheng Chen, Xiaohong Liu, Journal of Peptide Science, 2013

It has been reported that obestatin regulates adipocyte metabolism via receptors on the cell surface. We wondered whether obestatin can interact with intracellular components that activated signalling pathways in adipocytes. Because obestatin (human) only presents one lysine (at position 10), ...


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