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20.995 Aktuelle Fachpublikationen zum Thema Addition

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Structural and enzymatic analyses of a glucosyltransferase Alr3699/HepE involved in Anabaena heterocyst envelop polysaccharide biosynthesis

01.05.2016 | Xue-Ping Wang; Yong-Liang Jiang; Ya-Nan Dai; Wang Cheng; Yuxing Chen; Cong-Zhao Zhou, Glycobiology , 2016

Formation of the heterocyst envelope polysaccharide (HEP) is a key process for cyanobacterial heterocyst differentiation. The maturation of HEP in Anabaena sp. strain PCC 7120 is controlled by a gene cluster termed HEP island in addition to an operon alr3698-alr3699, which encodes two putative ...

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Structure and biological activity of a fucosylated chondroitin sulfate from the sea cucumber Cucumaria japonica

01.05.2016 | Nadezhda E Ustyuzhanina; Maria I Bilan; Andrey S Dmitrenok; Alexander S Shashkov; Mikhail I Kusaykin; Valentin A Sto ..., Glycobiology , 2016

A fucosylated chondroitin sulfate (FCS) was isolated from the body wall of Pacific sea cucumber Cucumaria japonica by extraction in the presence of papain followed by Cetavlon precipitation and anion-exchange chromatography. FCS was shown to contain d-GalNAc, d-GlcA, l-Fuc and sulfate in molar ...

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Urinary {beta}-galactosidase stimulates Ca2+ transport by stabilizing TRPV5 at the plasma membrane

01.05.2016 | Elizabeth H P Leunissen; Maxime G Blanchard; Fareeba Sheedfar; Marla Lavrijsen; Jenny van der Wijst; René J M Bindel ..., Glycobiology , 2016

Transcellular Ca2+ transport in the late distal convoluted tubule and connecting tubule (DCT2/CNT) of the kidney is a finely controlled process mediated by the transient receptor potential vanilloid type 5 (TRPV5) channel. A complex-type-N-glycan bound at the extracellular residue Asn358 of TRPV5 ...

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Biochemical characterization of an {alpha}1,2-colitosyltransferase from Escherichia coli O55:H7

01.05.2016 | Zhigang Wu; Guohui Zhao; Tiehai Li; Jingyao Qu; Wanyi Guan; Jiajia Wang; Cheng Ma; Xu Li; Wei Zhao; Peng G Wang; Lei Li, Glycobiology , 2016

Colitose, also known as 3,6-dideoxy-l-galactose or 3-deoxy-l-fucose, is one of only five naturally occurring 3,6-dideoxyhexoses. Colitose was found in lipopolysaccharide of a number of infectious bacteria, including Escherichia coli O55 & O111 and Vibrio cholera O22 & O139. To date, no ...

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The valine and lysine residues in the conserved FxVTxK motif are important for the function of phylogenetically distant plant cellulose synthases

01.05.2016 | Erin Slabaugh; Tess Scavuzzo-Duggan; Arielle Chaves; Liza Wilson; Carmen Wilson; Jonathan K Davis; Daniel J Cosgrove ..., Glycobiology , 2016

Cellulose synthases (CESAs) synthesize the β-1,4-glucan chains that coalesce to form cellulose microfibrils in plant cell walls. In addition to a large cytosolic (catalytic) domain, CESAs have eight predicted transmembrane helices (TMHs). However, analogous to the structure of BcsA, a bacterial ...

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Ripple-triggered stimulation of the locus coeruleus during post-learning sleep disrupts ripple/spindle coupling and impairs memory consolidation [RESEARCH]

01.05.2016 | Yulia Novitskaya; Susan J. Sara; Nikos K. Logothetis; Oxana Eschenko, Learning & Memory, 2016

Experience-induced replay of neuronal ensembles occurs during hippocampal high-frequency oscillations, or ripples. Post-learning increase in ripple rate is predictive of memory recall, while ripple disruption impairs learning. Ripples may thus present a fundamental component of a ...

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Involvement of phosphorylated Apis mellifera CREB in gating a honeybee's behavioral response to an external stimulus [RESEARCH]

01.05.2016 | Katrin B. Gehring; Karin Heufelder; Janina Feige; Paul Bauer; Yan Dyck; Lea Ehrhardt; Johannes Kühnemund; Anja Bergm ..., Learning & Memory, 2016

The transcription factor cAMP-response element-binding protein (CREB) is involved in neuronal plasticity. Phosphorylation activates CREB and an increased level of phosphorylated CREB is regarded as an indicator of CREB-dependent transcriptional activation. In honeybees (Apis mellifera) we ...

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Human Lineage-Specific Transcriptional Regulation through GA-Binding Protein Transcription Factor Alpha (GABPa)

01.05.2016 | Alvaro Perdomo-Sabogal; Katja Nowick; Ilaria Piccini; Ralf Sudbrak; Hans Lehrach; Marie-Laure Yaspo; Hans-Jörg Warna ..., Molecular Biology and Evolution, 2016

A substantial fraction of phenotypic differences between closely related species are likely caused by differences in gene regulation. While this has already been postulated over 30 years ago, only few examples of evolutionary changes in gene regulation have been verified. Here, we identified and ...

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High-throughput sequencing of partially edited trypanosome mRNAs reveals barriers to editing progression and evidence for alternative editing [BIOINFORMATICS]

01.05.2016 | Rachel M. Simpson; Andrew E. Bruno; Jonathan E. Bard; Michael J. Buck; Laurie K. Read, RNA, 2016

Uridine insertion/deletion RNA editing in kinetoplastids entails the addition and deletion of uridine residues throughout the length of mitochondrial transcripts to generate translatable mRNAs. This complex process requires the coordinated use of several multiprotein complexes as well as the ...

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Trans and cis factors affecting A-to-I RNA editing efficiency of a noncoding editing target in C. elegans [REPORT]

01.05.2016 | Michael C. Washburn; Heather A. Hundley, RNA, 2016

Adenosine-to-inosine RNA editing by ADARs affects thousands of adenosines in an organism's transcriptome. However, adenosines are not edited at equal levels nor do these editing levels correlate well with ADAR expression levels. Therefore, additional mechanisms are utilized by the cell to dictate ...

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