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2.134 Aktuelle Fachpublikationen zum Thema Addition

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Structural and enzymatic analyses of a glucosyltransferase Alr3699/HepE involved in Anabaena heterocyst envelop polysaccharide biosynthesis

01.05.2016 | Xue-Ping Wang; Yong-Liang Jiang; Ya-Nan Dai; Wang Cheng; Yuxing Chen; Cong-Zhao Zhou, Glycobiology , 2016

Formation of the heterocyst envelope polysaccharide (HEP) is a key process for cyanobacterial heterocyst differentiation. The maturation of HEP in Anabaena sp. strain PCC 7120 is controlled by a gene cluster termed HEP island in addition to an operon alr3698-alr3699, which encodes two putative ...

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Structure and biological activity of a fucosylated chondroitin sulfate from the sea cucumber Cucumaria japonica

01.05.2016 | Nadezhda E Ustyuzhanina; Maria I Bilan; Andrey S Dmitrenok; Alexander S Shashkov; Mikhail I Kusaykin; Valentin A Sto ..., Glycobiology , 2016

A fucosylated chondroitin sulfate (FCS) was isolated from the body wall of Pacific sea cucumber Cucumaria japonica by extraction in the presence of papain followed by Cetavlon precipitation and anion-exchange chromatography. FCS was shown to contain d-GalNAc, d-GlcA, l-Fuc and sulfate in molar ...

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Urinary {beta}-galactosidase stimulates Ca2+ transport by stabilizing TRPV5 at the plasma membrane

01.05.2016 | Elizabeth H P Leunissen; Maxime G Blanchard; Fareeba Sheedfar; Marla Lavrijsen; Jenny van der Wijst; René J M Bindel ..., Glycobiology , 2016

Transcellular Ca2+ transport in the late distal convoluted tubule and connecting tubule (DCT2/CNT) of the kidney is a finely controlled process mediated by the transient receptor potential vanilloid type 5 (TRPV5) channel. A complex-type-N-glycan bound at the extracellular residue Asn358 of TRPV5 ...

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Biochemical characterization of an {alpha}1,2-colitosyltransferase from Escherichia coli O55:H7

01.05.2016 | Zhigang Wu; Guohui Zhao; Tiehai Li; Jingyao Qu; Wanyi Guan; Jiajia Wang; Cheng Ma; Xu Li; Wei Zhao; Peng G Wang; Lei Li, Glycobiology , 2016

Colitose, also known as 3,6-dideoxy-l-galactose or 3-deoxy-l-fucose, is one of only five naturally occurring 3,6-dideoxyhexoses. Colitose was found in lipopolysaccharide of a number of infectious bacteria, including Escherichia coli O55 & O111 and Vibrio cholera O22 & O139. To date, no ...

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The valine and lysine residues in the conserved FxVTxK motif are important for the function of phylogenetically distant plant cellulose synthases

01.05.2016 | Erin Slabaugh; Tess Scavuzzo-Duggan; Arielle Chaves; Liza Wilson; Carmen Wilson; Jonathan K Davis; Daniel J Cosgrove ..., Glycobiology , 2016

Cellulose synthases (CESAs) synthesize the β-1,4-glucan chains that coalesce to form cellulose microfibrils in plant cell walls. In addition to a large cytosolic (catalytic) domain, CESAs have eight predicted transmembrane helices (TMHs). However, analogous to the structure of BcsA, a bacterial ...

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Human Lineage-Specific Transcriptional Regulation through GA-Binding Protein Transcription Factor Alpha (GABPa)

01.05.2016 | Alvaro Perdomo-Sabogal; Katja Nowick; Ilaria Piccini; Ralf Sudbrak; Hans Lehrach; Marie-Laure Yaspo; Hans-Jörg Warna ..., Molecular Biology and Evolution, 2016

A substantial fraction of phenotypic differences between closely related species are likely caused by differences in gene regulation. While this has already been postulated over 30 years ago, only few examples of evolutionary changes in gene regulation have been verified. Here, we identified and ...

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Contrasting Frequencies and Effects of cis- and trans-Regulatory Mutations Affecting Gene Expression

01.05.2016 | Brian P. H. Metzger; Fabien Duveau; David C. Yuan; Stephen Tryban; Bing Yang; Patricia J. Wittkopp, Molecular Biology and Evolution, 2016

Heritable differences in gene expression are caused by mutations in DNA sequences encoding cis-regulatory elements and trans-regulatory factors. These two classes of regulatory change differ in their relative contributions to expression differences in natural populations because of the combined ...

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Robust Estimation of Evolutionary Distances with Information Theory

01.05.2016 | Minh Duc Cao; Lloyd Allison; Trevor I. Dix; Mikael Bodén, Molecular Biology and Evolution, 2016

Methods for measuring genetic distances in phylogenetics are known to be sensitive to the evolutionary model assumed. However, there is a lack of established methodology to accommodate the trade-off between incorporating sufficient biological reality and avoiding model overfitting. In addition, ...

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Transcriptional, post-transcriptional and chromatin-associated regulation of pri-miRNAs, pre-miRNAs and moRNAs

20.04.2016 | Chirag Nepal; Marion Coolen; Yavor Hadzhiev; Delphine Cussigh; Piotr Mydel; Vidar M. Steen; Piero Carninci; Jesper B ..., Nucleic Acids Research, 2016

MicroRNAs (miRNAs) play a major role in the post-transcriptional regulation of target genes, especially in development and differentiation. Our understanding about the transcriptional regulation of miRNA genes is limited by inadequate annotation of primary miRNA (pri-miRNA) transcripts. Here, we ...

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PolDIP2 interacts with human PrimPol and enhances its DNA polymerase activities

20.04.2016 | Thomas A. Guilliam; Laura J. Bailey; Nigel C. Brissett; Aidan J. Doherty, Nucleic Acids Research, 2016

Translesion synthesis (TLS) employs specialized DNA polymerases to bypass replication fork stalling lesions. PrimPol was recently identified as a TLS primase and polymerase involved in DNA damage tolerance. Here, we identify a novel PrimPol binding partner, PolDIP2, and describe how it regulates ...

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