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Polyion Complex Micelle Based on Albumin–Polymer Conjugates: Multifunctional Oligonucleotide Transfection Vectors for Anticancer Chemotherapeutics

16.10.2014 | Yanyan Jiang; Hongxu Lu; Yee Yee Khine; Aydan Dag; Martina H. Stenzel, Biomacromolecules, 2014

Novel biocompatible polyion complex micelles, containing bovine serum albumin (BSA), polymer, and oligonucleotide, were synthesized as a generation of vectors for the gene transfection. Maleimide-terminated poly((N,N-dimethyl amino) ethyl methacrylate) (PDMAEMA) was prepared via reversible ...


Analysis of G-Block Distributions and Their Impact on Gel Properties of in Vitro Epimerized Mannuronan

03.09.2013 | Olav Aarstad; Berit Løkensgard Strand; Lise Mari Klepp-Andersen; Gudmund Skjåk-Bræk, Biomacromolecules, 2013

This paper reports a study of the distribution and function of homopolymeric guluronic acid blocks (G-blocks) in enzymatically modified alginate. High molecular weight mannuronan was incubated with one native (AlgE6) and two engineered G-block generating mannuronan C-5 epimerases (AlgE64 and ...


Location of the Bacteriophage P22 Coat Protein C-Terminus Provides Opportunities for the Design of Capsid-Based Materials

27.08.2013 | Amy Servid; Paul Jordan; Alison O’Neil; Peter Prevelige; Trevor Douglas, Biomacromolecules, 2013

Rational design of modifications to the interior and exterior surfaces of virus-like particles (VLPs) for future therapeutic and materials applications is based on structural information about the capsid. Existing cryo-electron microscopy-based models suggest that the C-terminus of the ...


Terminal-Functionality Effect of Poly(N-isopropylacrylamide) Brush Surfaces on Temperature-Controlled Cell Adhesion/Detachment

23.08.2013 | Naoki Matsuzaka; Masamichi Nakayama; Hironobu Takahashi; Masayuki Yamato; Akihiko Kikuchi; Teruo Okano, Biomacromolecules, 2013

Terminally functionalized poly(N-isopropylacrylamide) (PIPAAm) brush grafted glass surfaces were prepared by a surface-initiated reversible addition-fragmentation chain transfer radical (SI-RAFT) polymerization. SI-RAFT mediated PIPAAm chains possessed terminal dodecyl trithiocarbonate groups ...


Stealth Polymeric Vesicles via Metal-Free Click Coupling

21.08.2013 | Michael J. Isaacman; Eleonora M. Corigliano; Luke S. Theogarajan, Biomacromolecules, 2013

The strain-promoted azide–alkyne cycloaddition represents an optimal metal-free method for the modular coupling of amphiphilic polymer blocks. Hydrophilic poly(oxazoline) (PMOXA) or poly(ethylene glycol) (PEG) A-blocks were coupled with a hydrophobic poly(siloxane) B-block to provide triblock ...


3D Culture of Adipose-Tissue-Derived Stem Cells Mainly Leads to Chondrogenesis in Poly(ethylene glycol)-Poly(l-alanine) Diblock Copolymer Thermogel

19.08.2013 | Bora Yeon; Min Hee Park; Hyo Jung Moon; Seung-Jin Kim; Young Woo Cheon; Byeongmoon Jeong, Biomacromolecules, 2013

Poly(ethylene glycol)-b-poly(l-alanine) (PEG-L-PA)s with L-PA molecular weights of 620, 1100, and 2480 Da and a fixed molecular weight of PEG at 5000 Da were synthesized to compare the thermosensitive behavior, and to investigate their potential as a three-dimensional (3D) culture matrix of ...


Versatile Functional Microstructured Polystyrene-Based Platforms for Protein Patterning and Recognition

19.08.2013 | Marta Palacios-Cuesta; Aitziber L. Cortajarena; Olga García; Juan Rodríguez-Hernández, Biomacromolecules, 2013

We report the preparation of different functional surface patterns based on the optimization of the photo-cross-linking/degradation kinetics of polystyrene (PS) upon exposure to UV-light. We employed a PS-b-PGA (polystyrene-block-poly(l-glutamic acid)) block copolymer that will, in addition to ...


Split of Chiral Degeneracy in Mechanical and Structural Properties of Oligopeptide–Polysaccharide Biomaterials

08.08.2013 | Marc B. Taraban; Laura L. Hyland; Y. Bruce Yu, Biomacromolecules, 2013

Enantiomeric biomaterials which are mirror images of each other are characterized by chiral degeneracy—identical structural characteristics and bulk material properties. The addition of another chiral component, d-polysaccharide, has been shown to split such degeneracy and result in two distinct ...


Transfection Efficiency of DNA Enhanced by Association with Salt-Free Catanionic Vesicles

01.08.2013 | Lu Xu; Lei Feng; Renhao Dong; Jingcheng Hao; Shuli Dong, Biomacromolecules, 2013

The interaction of DNA with salt-free tetradecyltrimethylammonium hydroxide and lauric acid lamellar vesicles with positive charges was investigated to probe potential applications of vesicles in DNA transfection. The aggregation morphology of the vesicles changes greatly with the addition of DNA ...


Fluorescence Characterization of Co-immobilization-Induced Multi-Enzyme Aggregation in a Polymer Matrix Using Förster Resonance Energy Transfer (FRET): Toward the Metabolon Biomimic

29.07.2013 | Fei Wu; Shelley D. Minteer, Biomacromolecules, 2013

Sequential metabolic enzymes can form supramolecular complexes named metabolons in vivo through enzyme–enzyme association or aggregation to facilitate efficient substrate channeling. By separately labeling enzymes with lysine-targeting carboxylic acid succinimidyl ester fluorophores of distinct ...


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