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Deficiency of {alpha}-glucosidase I alters glycoprotein glycosylation and lifespan in Caenorhabditis elegans

01.10.2013 | Toshihiko Katoh; Juri Takase; Yasushi Tani; Ryuta Amamoto; Naofumi Aoshima; Michael Tiemeyer; Kenji Yamamoto; Hisash ..., Glycobiology , 2013

Endoplasmic reticulum (ER) α-glucosidase I is an enzyme that trims the distal α1,2-linked glucose (Glc) residue from the Glc3Man9GlcNAc2 oligosaccharide following its addition to nascent glycoproteins in the initial step of processing. This reaction is critical to the subsequent processing of ...


Fucosyltransferase VII improves the function of selectin ligands on cord blood hematopoietic stem cells

01.10.2013 | Xiang Wan; Hidetaka Sato; Hiromasa Miyaji; J Michael McDaniel; Yuesi Wang; Etsuji Kaneko; BreeAnna Gibson; Padmaja M ..., Glycobiology , 2013

Selectins and their carbohydrate ligands mediate the homing of hematopoietic stem/progenitor cells (HSPCs) to the bone marrow. We have previously shown that ex vivo fucosylation of selectin ligands on HSPCs by α1,3 fucosyltransferase VI (FUT6) leads to improved human cord blood (CB)-HSPC ...


An integrated toolkit for accurate prediction and analysis of cis-regulatory motifs at a genome scale

15.09.2013 | Qin Ma; Bingqiang Liu; Chuan Zhou; Yanbin Yin; Guojun Li; Ying Xu, Bioinformatics, 2013

Motivation: We present an integrated toolkit, BoBro2.0, for prediction and analysis of cis-regulatory motifs. This toolkit can (i) reliably identify statistically significant cis-regulatory motifs at a genome scale; (ii) accurately scan for all motif instances of a query motif in specified ...


pyRMSD: a Python package for efficient pairwise RMSD matrix calculation and handling

15.09.2013 | Víctor A. Gil; Víctor Guallar, Bioinformatics, 2013

Summary: We introduce pyRMSD, an open source standalone Python package that aims at offering an integrative and efficient way of performing Root Mean Square Deviation (RMSD)-related calculations of large sets of structures. It is specially tuned to do fast collective RMSD calculations, as ...


Effect of TAT‐obestatin on proliferation, differentiation, apoptosis and lipolysis in 3T3‐L1 preadipocytes

14.09.2013 | Guangcai Ren, Zuyong He, Peiqing Cong, Jingwei Yu, Yufeng Qin, Yaosheng Chen, Xiaohong Liu, Journal of Peptide Science, 2013

It has been reported that obestatin regulates adipocyte metabolism via receptors on the cell surface. We wondered whether obestatin can interact with intracellular components that activated signalling pathways in adipocytes. Because obestatin (human) only presents one lysine (at position 10), ...


Hardfats as crystallization modifiers of cocoa butter

13.09.2013 | Ana Paula Badan Ribeiro, Rodrigo Corrêa Basso, Adenilson Oliveira dos Santos, Guilherme Calligaris de Andrade, Lisan ..., European Journal of Lipid Science and Technology, 2013

Hardfats, or fully hydrogenated oils, consist of materials with homogeneous composition, composed by high melting point triacylglycerols. Hardfats are regarded as relatively new materials, and remain unexplored in lipid technology, notwithstanding being low‐cost industrial products. They can ...


Clinical significance of de novo and inherited copy number variation

13.09.2013 | Anneke T. Vulto‐van Silfhout, Jayne Y. Hehir‐Kwa, Bregje W.M. Bon, Janneke H.M. Schuurs‐Hoeijmakers, Stephen Meader, ..., Human Mutation, 2013

ABSTRACT Copy number variations (CNVs) are a common cause of intellectual disability and/or multiple congenital anomalies (ID/MCA). However, the clinical interpretation of CNVs remains challenging, especially for inherited CNVs. 5,531 well‐phenotyped patients with ID/MCA were screened for rare ...


Proteomic characterization of the acid tolerance response in Lactobacillus delbrueckii subsp. bulgaricus CAUH1 and functional identification of a novel acid stress‐related transcriptional regulator Ldb0677

13.09.2013 | Zhengyuan Zhai, François P. Douillard, Haoran An, Guohong Wang, Xinghua Guo, Yunbo Luo, Yanling Hao, Environmental Microbiology, 2013

Summary To overcome the deleterious effects of acid stress, L. bulgaricus elicits an adaptive response to acid stress. In this study, proteomics approach complemented by transcriptional analysis revealed some cellular changes in L. bulgaricus CAUH1 during acid adaptation. We observed an ...


Metabolic versatility of toluene‐degrading, iron‐reducing bacteria in tidal flat sediment, characterized by stable isotope probing‐based metagenomic analysis

13.09.2013 | So‐Jeong Kim, Soo‐Je Park, In‐Tae Cha, Deullae Min, Jin‐Seog Kim, Won‐Hyung Chung, Jong‐Chan Chae, Che Ok Jeon, Sung ..., Environmental Microbiology, 2013

Summary DNA stable isotope probing and metagenomic sequencing were used to assess the metabolic potential of iron‐reducing bacteria involved in anaerobic aromatic hydrocarbon degradation in oil spill‐affected tidal flats. In a microcosm experiment, 13C‐toluene was degraded with the ...


Reactive oxygen species production induced by ethanol in Saccharomyces cerevisiae increases because of a dysfunctional mitochondrial iron‐sulfur cluster assembly system

12.09.2013 | Rocio Viridiana Pérez‐Gallardo, Luis Sánchez Briones, Alma Laura Díaz‐Pérez, Sergio Gutiérrez, José Salud Rodríguez‐ ..., FEMS Yeast Research, 2013

Abstract Ethanol accumulation during fermentation contributes to the toxic effects in Saccharomyces cerevisiae, impairing its viability and fermentative capabilities. The iron sulfur (Fe‐S) cluster biogenesis is encoded by the ISC genes. ROS generation is associated with iron release from Fe‐S ...


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