01.05.2013 | Clare Gooding; Christopher Edge; Mike Lorenz; Miguel B. Coelho; Mikael Winters; Clemens F. Kaminski; Dmitry Cherny; ..., Nucleic Acids Research, 2013

Exon 3 of the rat α-tropomyosin (Tpm1) gene is repressed in smooth muscle cells, allowing inclusion of the mutually exclusive partner exon 2. Two key types of elements affect repression of exon 3 splicing: binding sites for polypyrimidine tract-binding protein (PTB) and additional negative ...

mehr

01.05.2013 | José P. Afonso; Kiran Chintakayala; Chatrudee Suwannachart; Svetlana Sedelnikova; Kevin Giles; John B. Hoyes; Panos ..., Nucleic Acids Research, 2013

The clamp-loader complex plays a crucial role in DNA replication by loading the β-clamp onto primed DNA to be used by the replicative polymerase. Relatively little is known about the stoichiometry, structure and assembly pathway of this complex, and how it interacts with the replicative ...

mehr

01.05.2013 | Anne Katrine Alsing; Kim Sneppen, Nucleic Acids Research, 2013

The olfactory system integrates signals from receptors expressed in olfactory sensory neurons. Each sensory neuron expresses only one of many similar olfactory receptors (ORs). The choice of receptor is made stochastically early in the differentiation process and is maintained throughout the ...

mehr

01.05.2013 | Tong Wang; Yizhi Cui; Jingjie Jin; Jiahui Guo; Guibin Wang; Xingfeng Yin; Qing-Yu He; Gong Zhang, Nucleic Acids Research, 2013

As a well-known phenomenon, total mRNAs poorly correlate to proteins in their abundances as reported. Recent findings calculated with bivariate models suggested even poorer such correlation, whereas focusing on the translating mRNAs (ribosome nascent-chain complex-bound mRNAs, RNC-mRNAs) ...

mehr

01.05.2013 | Purba Mukherjee; Indrajit Lahiri; Janice D. Pata, Nucleic Acids Research, 2013

Polymerases belonging to the DinB class of the Y-family translesion synthesis DNA polymerases have a preference for accurately and efficiently bypassing damaged guanosines. These DinB polymerases also generate single-base (–1) deletions at high frequencies with most occurring on repetitive ...

mehr

01.05.2013 | Katrin Schneider; Christiane Fuchs; Akos Dobay; Andrea Rottach; Weihua Qin; Patricia Wolf; José M. Álvarez-Castro; M ..., Nucleic Acids Research, 2013

DNA methyltransferase 1 (Dnmt1) reestablishes methylation of hemimethylated CpG sites generated during DNA replication in mammalian cells. Two subdomains, the proliferating cell nuclear antigen (PCNA)-binding domain (PBD) and the targeting sequence (TS) domain, target Dnmt1 to the ...

mehr

01.04.2013 | Kartik Soni; Ashwani Choudhary; Ashok Patowary; Angom Ramcharan Singh; Shipra Bhatia; Sridhar Sivasubbu; Shanti Chan ..., Nucleic Acids Research, 2013

MicroRNAs (miRNAs) are small, endogenous, regulatory RNA molecules that can bind to partially complementary regions on target messenger RNAs and impede their expression or translation. We rationalized that miRNAs, being localized to the cytoplasm, will be maternally inherited during ...

mehr

01.04.2013 | Kyoung-Jae Won; Xian Zhang; Tao Wang; Bo Ding; Debasish Raha; Michael Snyder; Bing Ren; Wei Wang, Nucleic Acids Research, 2013

Epigenetic regulation is dynamic and cell-type dependent. The recently available epigenomic data in multiple cell types provide an unprecedented opportunity for a comparative study of epigenetic landscape. We developed a machine-learning method called ChroModule to annotate the epigenetic ...

mehr

01.04.2013 | Timothy J. Mead; Qiuqing Wang; Pallavi Bhattaram; Peter Dy; Solomon Afelik; Jan Jensen; Véronique Lefebvre, Nucleic Acids Research, 2013

SOX9 encodes a transcription factor that presides over the specification and differentiation of numerous progenitor and differentiated cell types, and although SOX9 haploinsufficiency and overexpression cause severe diseases in humans, including campomelic dysplasia, sex reversal and cancer, ...

mehr

01.04.2013 | Anthony Mathelier; Alessandra Carbone, Nucleic Acids Research, 2013

MicroRNAs (miRNAs) can group together along the human genome to form stable secondary structures made of several hairpins hosting miRNAs in their stems. The few known examples of such structures are all involved in cancer development. A large scale computational analysis of human chromosomes ...

mehr